论文部分内容阅读
目的观察高氟染毒新西兰白兔某些氧化、抗氧化和血管功能指标的变化。方法 20只雄性新西兰白兔随机分为对照组,饮去离子水,饲基础饲料;高脂组,饮去离子水,饲基础饲料加0.5%胆固醇、7%蛋黄粉高脂饲料;高氟组,饮含氟质量浓度100mg/L高氟水,饲基础饲料;高氟高脂组,饮含氟质量浓度100mg/L高氟水,饲基础饲料加0.5%胆固醇、7%蛋黄粉高脂饲料,实验期6个月。于实验前、实验3个月、6个月取血氟离子电极法测血氟含量。实验6个月取血、心和肝制备组织匀浆,生化法检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)水平;放免法检测血浆6-酮-前列腺F1α(6-keto-PGFlα)、血栓素B2(TXB2)和内皮素-1(ET-1);生化法检测血清一氧化氮合酶(NOS)、诱导型NOS(iNOS)、内皮型NOS(eNOS)活性;原位杂交法检测白细胞iNOS-mRNA和eNOS-mRNA表达。结果新西兰白兔饮用高氟水3个月、6个月时血清氟升高;6个月时血液、肝和心肌SOD、GSH-Px活性降低(P<0.01,P<0.05)、心肌MDA含量升高(P<0.05);血浆6-keto-PGFlα含量下降(P<0.01),TXB2和ET-1含量升高(P<0.01,P<0.05);血清总NOS活性下降(P<0.05),肝总NOS活力、心肌和肝iNOS活性升高(P<0.05);血白细胞iNOS-mRNA表达增强,eNOS-mRNA表达减弱(P<0.05)。析因方差分析,血清、肝和心肌SOD活性及血清MDA含量,血浆ET-1含量以及血清iNOS活性、肝总NOS活性等指标高氟与高脂具有交互增强作用(P<0.01,P<0.05)。结论高氟抑制抗氧化酶,血管内皮功能受损,机体NO代谢紊乱,此过程高氟与高脂具有一定的协同作用。
Objective To observe the changes of some oxidative, anti-oxidative and vascular function indexes in high-fluorine-exposed New Zealand white rabbits. Methods Twenty male New Zealand white rabbits were randomly divided into control group, drinking deionized water, basic diet, high fat diet group, drinking deionized water, basal diet supplemented with 0.5% cholesterol and 7% , Drinking high fluoride water containing 100mg / L fluoride, feed basal diet; high fluoride and high fat group, drinking fluoride containing 100mg / L fluoride water, basal diet plus 0.5% cholesterol, 7% , The experimental period of 6 months. Before the experiment, the experiment 3 months, 6 months to take blood fluoride ion electrode method to measure blood fluoride content. Six months after the experiment, blood, heart and liver tissue were homogenized, and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) Serum levels of 6-keto-PGFlα, TXB2 and ET-1 were detected by radioimmunoassay (RIA). Serum levels of nitric oxide synthase (NOS) NOS (iNOS) and endothelial NOS (eNOS) activity were detected by immunohistochemistry. The expressions of iNOS-mRNA and eNOS-mRNA in leukocytes were detected by in situ hybridization. Results New Zealand white rabbits were fed with high fluoride water for 3 months and serum fluoride increased at 6 months. The activities of SOD, GSH-Px in blood, liver and myocardium were decreased (P <0.01, P <0.05) (P <0.01, P <0.05). The content of 6-keto-PGFlα in plasma decreased (P <0.01), TXB2 and ET-1 levels increased (P <0.05). The expression of iNOS mRNA in leukocytes increased and the expression of eNOS mRNA decreased (P <0.05). Factor analysis of variance, SOD activity in serum, liver and myocardium and serum MDA content, plasma ET-1 content and serum iNOS activity, total liver NOS activity and other indicators of high fluoride and high fat interaction enhanced (P <0.01, P <0.05 ). Conclusion High fluoride inhibits the activity of antioxidant enzymes and endothelial dysfunction, and the metabolism of NO in the body is disordered. In this process, high fluoride has some synergistic effects with high fat.