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以银杏的胚为材料诱导愈伤组织产生,研究愈伤组织生长量及其培养过程中过氧化物酶(POD)活性和ATP含量的变化,结果表明,2,4-D与BA组合比NAA和KT有利于各种外植体愈伤组织的诱导,NAA与KT组合更利于愈伤的继代培养。未成熟胚子叶愈伤组织的生长曲线大致为“S”型,在含BA的培养基上,愈伤组织前期生长较快,而在后期(24~28 d)鲜重有所下降,POD活性和ATP含量在20 d时达到最大;在含KT培养基上,愈伤组织在24~28 d期间仍有增长,其POD活性和ATP含量在28 d时最大,且有继续上升的趋势。这表明POD活性和ATP含量与银杏愈伤组织的生长有一定的相关性。
The embryo of Ginkgo biloba was used as the material to induce the callus formation. The growth of callus and the changes of peroxidase (POD) activity and ATP content during the culture were studied. The results showed that the ratio of 2,4-D to BA was higher than that of NAA And KT are conducive to the induction of callus of various explants. The combination of NAA and KT is more conducive to callus subculture. The callus growth curve of immature embryo leaf was roughly “S” type. The callus grew faster in the medium containing BA, while the fresh weight decreased in late stage (24-28 d) POD activity and ATP content reached the maximum at 20 days. On KT medium, the callus still grew during the period of 24-28 days, and its POD activity and ATP content reached the maximum at 28th day and continued to increase . This indicates that POD activity and ATP content have some correlation with the growth of Ginkgo callus.