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中华猕猴桃由于有产量高、寿命长、营养丰富的特点,因而获有仙果、长生果的美名。但由于猕猴桃种子繁殖的后代易发生分离,无法直接利用种子繁殖良种株系。初选出的优良单株枝条数量有限,用嫁接和扦插的方法,一时也难于繁殖出大批种苗。试验证明,运用组织培养新技术,既能加快繁殖优良单株的速度,又能保持良种特性。我们从1983年3月至1984年11月底,进行了中华猕猴桃试管苗培育和栽培技术的研究,现介绍如下。一、愈伤组织的诱导1982年10月,我们将四川省苍溪县三溪口林场和华云山提供的中华猕猴桃硬毛82—7枝条移入温室,1983年3月,剪取新长出的上部
Kiwifruit due to a high yield, long life, nutrient-rich features, which have cents fruit, fruit of the name of the United States. However, due to the offspring of kiwifruit seed breeding prone to separation, can not directly use seed propagation lines. First elected a limited number of fine branches per plant, with grafting and cutting methods, sometimes it is difficult to breed a large number of seedlings. Experiments show that the use of tissue culture of new technologies, both to speed up the reproduction of fine plants, but also to maintain the characteristics of elite. We from March 1983 to the end of November 1984, carried out the Chinese kiwifruit plantlets in vitro culture and cultivation techniques, are presented below. I. Callus Induction In October 1982, we transferred 82-7 branches of Chinese goosefoot bristles from Sanxiokou Forest Farm and Huayunshan Mountain in Cangxi County in October 1982 to the greenhouse. In March 1983, we cut the newly grown Upper part