论文部分内容阅读
提出了一种新的利用基于表面等离子体共振(Surface Plasmon Resonance,SPR)的生物光学传感器检测多种残留物的方法.引入分子标记技术,在修饰有羧甲基葡聚糖的芯片表面固定一层抗分子标记物的抗体,通过对待测样品的两步孵育,将对多种残留物的检测转化为对同一标记物的测量.实验采用的分子标记物为牛血清白蛋白(Bovine Serum Albumin,BSA),分别标记了四种被测物:卡那霉素、三聚氰胺、氨苄青霉素和链霉素.对四种被测物的水溶液进行了检测,检测限分别为50,10,1.25和10ng/mL,均低于各自的最大残留检出限(Maximum Residue Limit,MRL).该检测方法解决了原有竞争抑制法在检测时传感器的专一性问题,实现了传感器的通用性,同时该方法可推广到其他免疫法检测的领域,使免疫传感器、免疫试纸等通用性增强.
A new method for detecting various residues using biosensor based on Surface Plasmon Resonance (SPR) was proposed.The molecular tagging technique was used to immobilize the immobilized carboxymethyl dextran on the surface of the chip Layer anti-molecular marker antibody, the detection of multiple residues is converted to the measurement of the same marker by two-step incubation of the test sample.The molecular marker used in the experiment is Bovine Serum Albumin BSA), four kinds of analytes were labeled respectively: kanamycin, melamine, ampicillin and streptomycin.The aqueous solutions of four analytes were detected with detection limits of 50, 10, 1.25 and 10 ng / mL, respectively, which are lower than their respective Maximum Residue Limit (MRL) .The detection method solves the problem of the specificity of the sensor in the detection of the original competitive inhibition method, and realizes the versatility of the sensor, meanwhile, the method Can be extended to other areas of immunoassay detection, immunosensors, immune test strips and other generic enhancements.