,Choline Transporters in Human Lung Adenocarcinoma: Expression and Functional Implications

• 作 者: Tao WANG Jinjun LI Fei CHEN Yingjun ZHAO Xianghuo HE Dafang WAN Jianren GU
• 机 构: Medical College of Fudan University, Shanghai 200032, China“,”National Laboratory for Oncogenes and
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2007年9期
• 关键词: choline transporter lung adenocarcinoma proliferation CTL1

Choline is an essential nutrient for cell survival and proliferation, however, the expression and function of choline transporters have not been well identified in cancer. In this study, we detected the mRNA and protein expression of organic cation transporter OCT3, caitine/cation transporters OCTN 1 and OCTN2,and choline transporter-like protein CTL1 in human lung adenocarcinoma cell lines A549, H1299 and SPC-A-1.Their expression patte was further confirmed in 25 human primary adenocarcinoma tissues. The choline uptake in these cell lines was significantly blocked by CTL1 inhibitor, but only partially inhibited by OCT or OCTN inhibitors. The efficacy of these inhibitors on cell proliferation is closely correlated with their abilities to block choline transport. Under the native expression of these transporters, the total choline uptake was notably blocked by specific PI3K/AKT inhibitors. These results describe the expression of choline transporters and their relevant function in cell proliferation of human lung adenocarcinoma, thus providing a potential"choline-starvation" strategy of cancer interference through targeting choline transporters, especially CTL1.