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目的探讨非神经性烟碱型乙酰胆碱受体(α7nicotinic acetylcholine receptor,α7nAChR)在直肠癌组织中的表达及临床意义。方法直肠癌患者72例,取其手术切除直肠癌组织及癌旁组织标本,采用免疫组织化学SP法检测α7nAChR阳性表达率,Spearman法分析α7nAChR阳性表达与临床病理特征的相关性。结果肿瘤组织α7nAChR阳性表达率(69.4%)高于癌旁组织(16.7%)(P<0.05);α7nAChR阳性表达率在肿瘤Dukes分期A+B期者(62.0%)低于C+D期者(86.4%),高分化+中分化者(60.4%)低于低分化+未分化者(87.5%),肿瘤直径<5cm者(63.0%)低于≥5cm者(80.8%)(P<0.05);Spearman相关分析结果显示,Dukes分期C+D期、低分化+未分化、肿瘤直径≥5cm与α7nAChR阳性表达呈明显正相关(r=0.553,P=0.021;r=0.489,P=0.032;r=0.533,P=0.029)。结论α7nAChR在直肠癌组织中呈高表达,且其阳性表达与原发病灶低分化+未分化、肿瘤直径≥5cm、Ducks分期C+D期明显相关。
Objective To investigate the expression of non-neuronal nicotinic acetylcholine receptor (α7nAChR) in rectal cancer and its clinical significance. Methods Totally 72 rectal cancer patients were selected for surgical resection of rectal cancer tissues and adjacent tissues. The positive rate of α7 nAChR expression was detected by immunohistochemical SP method. The correlation between the expression of α7 nAChR and clinicopathological features was analyzed by Spearman method. Results The positive expression rate of α7nAChR in tumor was higher than that in paracancerous tissues (69.4% vs 16.7%, P <0.05). The positive rate of α7nAChR was lower in Dukes stage A + B (62.0%) than in C + D (86.4%), well differentiated + moderately differentiated (60.4%) lower than poorly differentiated + undifferentiated (87.5%), less than 5 cm (63.8% ); Spearman correlation analysis showed that positive expression of α7nAChR in Dukes stage C + D stage, poorly differentiated + undifferentiated, tumor diameter ≥5cm was positively correlated with positive (P <0.05) r = 0.533, P = 0.029). Conclusions α7nAChR is highly expressed in rectal cancer tissues. The positive expression of α7nAChR is correlated with poorly differentiated and undifferentiated primary tumors, with a diameter of 5cm or more, Ducks’ stage C + D.