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作者在埃塞俄比亚研究了81名界线类麻风病人临床、病理组织学特征与淋巴细胞转化试验(简称LTT)的关系。病人外周血液经聚蔗糖——泛影葡胺离心法分离淋巴细胞,加麻风菌抗原后培养7天,最后16小时加入~3H标记的胸腺嘧啶,回收时用液体闪烁计测定掺入同位素的数量。麻风菌抗原有两种,均自未治疗的瘤型麻风病人皮下结节取材制备。结节剪碎后置玻璃匀浆管中加入冷的含1％人血清白蛋白的磷酸缓冲液(简称PBS—HSA)研磨成匀浆,先低速(150g)离心5分钟,上清液再高速(2500g)离心1小时,沉渣用PBS—HSA洗二次,最后悬于PBS—HSA中使每毫升含抗酸菌1×10~9条,这种
The authors studied the clinical and histopathological features of 81 borderline leprosy patients in Ethiopia in relation to the lymphocyte transformation test (LTT). Lymphocytes were isolated from peripheral blood of patients by Ficoll-diatrizoate meglumine, and cultured with leprosy antigen for 7 days. 3H-labeled thymidine was added after the last 16 hours. The amount of isotopes incorporated into the blood was measured by liquid scintillator . There are two kinds of leprosy antigen, both from the untreated leprosy patients with subcutaneous nodules prepared. Nodular cut into the glass homogenizer after the tube was added cold containing 1% human serum albumin phosphate buffer (referred to as PBS-HSA) ground into a homogenate, first low-speed (150g) for 5 minutes, the supernatant then high speed (2500g) for 1 hour. The sediment was washed twice with PBS-HSA and finally suspended in PBS-HSA to make 1 × 10 ~ 9 antibacterial bacteria per ml.