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目的研究Th17细胞和调节性T细胞(Treg)及相关细胞因子在维吾尔族自身免疫性肝炎(AIH)患者外周血中的表达水平,探讨Th17细胞和Treg在AIH患者发病中的作用。方法应用流式细胞术检测AIH患者及正常对照者外周血Th17细胞和Treg的频数,ELISA检测血清中白细胞介素17(IL-17)和转化生长因子β(TGF-β)水平。结果与正常对照组相比,AIH患者外周血Th17细胞的频数明显增加、Treg频数明显降低,Th17细胞/Treg的比率明显增加;AIH患者外周血IL-17水平显著升高、而TGF-β明显降低,IL-17/TGF-β比值明显增加。结论 AIH患者外周血中Th17细胞/Treg及其相关细胞因子的比值增加。
Objective To investigate the expression of Th17 cells, regulatory T cells (Tregs) and related cytokines in the peripheral blood of patients with Uighur autoimmune hepatitis (AIH) and explore the role of Th17 cells and Tregs in the pathogenesis of AIH. Methods The frequency of Th17 cells and Tregs in peripheral blood of AIH patients and normal controls were detected by flow cytometry. The levels of IL-17 and TGF-β in serum were detected by ELISA. Results Compared with the normal control group, the frequency of Th17 cells in peripheral blood of AIH patients was significantly increased, the Treg frequency was significantly decreased and the ratio of Th17 cells / Tregs was significantly increased. The levels of IL-17 in peripheral blood of AIH patients were significantly increased, while TGF-β was significantly Decreased, IL-17 / TGF-β ratio increased significantly. Conclusion The ratio of Th17 cells / Treg and related cytokines in peripheral blood of patients with AIH is increased.