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目的研究葛根黄酮对辛基酚染毒雄性大鼠生殖系统的影响。方法将60只健康清洁级雄性SD大鼠按体重随机分为对照组(灌胃同体积的玉米油)、辛基酚低剂量染毒组(80mg/kg)、辛基酚中剂量染毒组(160mg/kg)、辛基酚高剂量染毒组(320mg/kg),低剂量干预组(0.5g/kg葛根黄酮+320mg/kg辛基酚),高剂量干预组(5g/kg葛根黄酮+320mg/kg辛基酚),每组10只。采用灌胃方式进行染毒,每周3次,先给大鼠灌入辛基酚溶液,2h后,再给干预组大鼠灌入等体积的葛根黄酮溶液,灌胃体积为5ml/kg,连续60d。染毒后,取出睾丸,进行形态学观察,并统计精子数、活动度和畸形率。结果与对照组比较,高、中、低剂量染毒组和高、低剂量干预组的精子数和活动度均较低,畸形率均较高,差异均有统计学意义(P<0.05)。与低剂量染毒组比较,高剂量染毒组的精子数和活动度均较低,差异有统计学意义(P<0.05);且随着辛基酚染毒剂量的升高,精子数和活动度呈下降趋势,畸形率呈上升趋势。与高剂量染毒组比较,高、低剂量干预组的精子活动度均较高,畸形率均较低,高剂量干预组的精子数较高,差异均有统计学意义(P<0.05)。结论葛根黄酮对辛基酚存在着一定的拮抗作用。
Objective To study the effects of Pueraria flavonoids on the reproductive system of male rats exposed to octylphenol. Methods Sixty healthy and clean male SD rats were randomly divided into control group (same volume of corn oil), octylphenol low dose group (80mg / kg), octylphenol medium dose group (160mg / kg), octylphenol high dose group (320mg / kg), low dose intervention group (0.5g / kg puerarin + 320mg / kg octylphenol) + 320mg / kg octylphenol), 10 per group. The rats were inoculated intraperitoneally with octylphenol solution three times a week. After 2 hours, the rats in the intervention group were infused with an equal volume of Pueraria flavonoids solution in a volume of 5 ml / kg, Continuous 60d. After exposure, the testis was removed for morphological observation, and statistics of sperm count, activity and deformity rate. Results Compared with the control group, the number of sperm and activity of high, medium and low dose exposure groups and high and low dose intervention groups were lower and the deformity rate was higher, the difference was statistically significant (P <0.05). Compared with the low dose exposure group, the sperm count and activity of the high dose exposure group were lower, the difference was statistically significant (P <0.05); and with the octylphenol dose increased, the number of sperm and Activity decreased, deformity showed an upward trend. Compared with the high-dose exposure group, the activity of sperm in high-dose and low-dose intervention groups were higher, the rate of deformity was lower, and the number of sperm in high-dose intervention group was higher, the difference was statistically significant (P <0.05). Conclusion Pueraria flavonoids octylphenol there is a certain antagonism.