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目的探讨孕期二苯基甲烷二异氰酸酯(MDI)暴露对子代雄性小鼠性成熟后睾丸组织能量代谢功能的影响。方法将清洁级健康昆明种孕小鼠16只按体重随机分为高剂量(1/8LD50)、中剂量(1/16LD50)、低剂量(1/32LD50)MDI染毒组和溶剂对照组(玉米油),每组4只。于妊娠第14天开始,每日灌胃染毒,灌胃剂量为5ml/kg,持续至母鼠自然分娩。记录孕鼠染毒期间体重增量和分娩时间。待仔鼠8周龄(性成熟)时,分离双侧睾丸,称重,并计算睾丸的脏器系数。并检测睾丸组织中琥珀酸脱氢酶(SDH)、Na-K-ATP酶、Ca-Mg-ATP酶和总ATP酶的活力以及蛋白的含量。结果各组孕鼠分娩时间和体重增量间比较,差异均无统计学意义(P>0.05)。与高剂量MDI染毒组比较,中、低剂量MDI染毒组和溶剂对照组睾丸系数较高,差异均有统计学意义(P<0.01或P<0.05)。与溶剂对照组比较,高、中、低剂量MDI染毒组SDH、Na-K-ATPase、Ca-Mg-ATPase以及总ATPase酶活力均下降,差异均有统计学意义(P<0.01或P<0.05);且随着染毒剂量的增高,SDH、Na-K-ATPase、Ca-Mg-ATPase以及总ATPase酶活力均呈下降趋势。结论孕期MDI暴露可干扰雄性子代小鼠睾丸的能量代谢功能,导致其性成熟后睾丸能量的产生和利用发生障碍。
Objective To investigate the effects of pregnant women exposure to diphenylmethane diisocyanate (MDI) on the energy metabolism of testes after sexual maturation in offspring male mice. Methods 16 healthy pregnant mice were randomly divided into high dose (1/8 LD50), middle dose (1/16 LD50), low dose (1/32 LD50) MDI and solvent control group (corn Oil), 4 per group. On the 14th day of gestation, the rats were intragastrically administered with a daily dose of 5ml / kg until the mice were delivered spontaneously. Record pregnant rats during exposure to weight gain and delivery time. When pups were 8 weeks old (sexual maturity), bilateral testis was isolated, weighed, and organ coefficients of testis were calculated. The activities and contents of succinate dehydrogenase (SDH), Na-K-ATPase, Ca-Mg-ATPase and total ATPase in testis tissue were detected. Results There was no significant difference in the time of delivery and weight gain between the two groups (P> 0.05). Compared with high dose MDI group, testis coefficient was higher in middle and low dose MDI group and solvent control group, the differences were statistically significant (P <0.01 or P <0.05). Compared with the solvent control group, the activities of SDH, Na-K-ATPase, Ca-Mg-ATPase and total ATPase in MDI group were significantly decreased (P <0.01 or P < 0.05). With the increase of exposure dose, the activity of SDH, Na-K-ATPase, Ca-Mg-ATPase and total ATPase decreased. Conclusion Exposure to MDI during pregnancy may interfere with the energy metabolism of testis in male offspring mice, leading to impairment of testicular energy production and utilization after sexual maturation.