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目的 :在利用基因芯片技术研究霍奇金氏淋巴瘤 (HL)及间变性大细胞淋巴瘤 (ALCL)细胞株凋亡相关基因的表达时发现caspase - 4在两者的mRNA表达水平存在明显差异的基础上 ,我们拟检测caspase - 4在HL及ALCL肿瘤组织中蛋白质水平的表达及试图探讨它们的表达与此两种淋巴瘤的发生机制的关系。方法 :选取临床手术切除、石蜡包埋的标本HL 18例、ALCL 15例 ,4 μm厚连续切片 ,HE染色及CD30 (Ber-H2 )、CD15 (C3D - 1)、CD2 0(L2 6 )、CD4 5RO(UCHL1)等免疫组织化学染色 ,通过形态学和免疫分型进行严格的病例筛选 ,确定诊断。切片经微波抗原修复 ,抗caspase - 4抗体 (SP法 )染片 ,DAB显色 ,光学显微镜高倍镜下计数阳性细胞。结果 :在ALCL组 ,15 /15例 (10 0 % )均高表达caspase - 4 ;而在HL病例组 16例 (88 8% )不表达、2例 (11 2 % )弱表达caspase - 4 ,两者表达差异显著 (P <0 0 1)。结论 :①Caspase - 4在两种淋巴瘤中的表达存在明显差异 ,即在ALCL的表达明显高于在HL的表达。Caspase- 4在HL和ALCL中表达的显著差异 ,提示两种肿瘤在发生发展的过程中存在不同的病理机制以及细胞凋亡信号转导途径受损部位不同。②Caspase - 4的表达特点有助于HL及ALCL的鉴别诊断。
AIM: To investigate the expression of caspase - 4 in both Hodgkin ’s lymphoma (HL) and Adenomatous Large Cell Lymphoma (ALCL) cell lines by gene chip technique. We intend to examine the expression of caspase - 4 protein in HL and ALCL tumor tissues and to explore their relationship with the pathogenesis of these two kinds of lymphomas. Methods: Seventy - five specimens of HL (18 cases), 15 cases of ALCL (4 cases) and paraffin - embedded specimens CD4 5RO (UCHL1) and other immunohistochemical staining, morphological and immunohistochemical strict screening of cases to determine the diagnosis. The sections were fixed with microwave antigen, stained with anti - caspase - 4 antibody (SP method), DAB colorimetric and counted under high power microscope under light microscope. Results: In ALCL group, caspase - 4 was highly expressed in 15 of 15 cases (100%), while in HL group, 16 cases (88.8%) did not express caspase - 4 in 2 cases (11.2% The difference was significant (P <0.01). Conclusion: (1) Caspase - 4 has obvious difference in the expression of two kinds of lymphoma, that is, the expression in ALCL is obviously higher than that in HL. The significant difference of Caspase-4 expression in HL and ALCL suggests that there are different pathological mechanisms in the development of both tumors and different parts of the apoptotic signal transduction pathway. The expression of Caspase - 4 contributes to the differential diagnosis of HL and ALCL.