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目的应用琼脂糖稀释法测定奈瑟淋球菌(NG)药物敏感性,并建立体外NG头孢曲松耐药株。方法配置含有不同浓度的四环素、环丙沙星、壮观霉素、头孢曲松培养基并接种一定量的NG菌悬液,培养24h后观察药敏试验结果。采用头孢曲松次抑菌浓度对NG敏感株体外反复诱导。结果 NG诱导前四环素、环丙沙星、壮观霉素、头孢曲松最小抑菌浓度(MIC)值分别为16μg/ml、16μg/ml、16μg/ml、0.016μg/ml。经过连续120次诱导传代,NG对四环素、环丙沙星、壮观霉素、头孢曲松MIC值分别为32μg/ml、16μg/ml、16μg/ml、1.00μg/ml,耐药子代经过30d的无药传代后,MIC值无明显变化。结论琼脂糖稀释法是测定奈NG药物敏感性的有效、实用的方法 ;通过体外人工诱导可获得稳定性好的NG耐头孢曲松株。
Objective To determine the drug sensitivity of Neisseria gonorrhoeae (NG) by agarose dilution method and establish a drug-resistant strain of ceftriaxone in vitro. Methods Tetracycline, ciprofloxacin, spectinomycin and ceftriaxone media with different concentrations were prepared and inoculated with a certain amount of NG suspension. After 24h incubation, the susceptibility test results were observed. The ceftriaxone secondary inhibitory concentration of NG-sensitive strains repeatedly induced in vitro. Results The minimum inhibitory concentration (MIC) of NG, tetracycline, ciprofloxacin, spectinomycin and ceftriaxone were 16μg / ml, 16μg / ml, 16μg / ml and 0.016μg / ml respectively. After 120 consecutive passages, the MIC values of NG against tetracycline, ciprofloxacin, spectinomycin and ceftriaxone were 32μg / ml, 16μg / ml, 16μg / ml and 1.00μg / ml, respectively. No drug passage, no significant changes in MIC values. Conclusion The agarose dilution method is an effective and practical method for the determination of drug sensitivity of Nao NG. A stable and stable strain of NG resistant to ceftriaxone can be obtained by in vitro induction.