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目的了解广西某监测点2007～2008年5岁以下腹泻住院儿童杯状病毒(HuCVs)腹泻流行状况和特点。方法收集广西某监测点5岁以下急性腹泻住院儿童患者粪便标本,用RT-PCR方法检测HuCVs,部分阳性标本进行序列测定和核苷酸序列比对、进化分析病毒型别。结果 2007～2008年HuCVs平均阳性检出率为13.3%(81/607),感染高峰出现在8～10月。58株HuCVs经序列测定比对分析,全部属于GII-4型,57株为Europe-2006b株相似株。结论广西杯状病毒腹泻感染高峰较其他地区提前,GII-4型/2006b株是2007～2008年广西某监测点HuCVs腹泻的优势流行株,应对广西婴幼儿杯状病毒腹泻进行长期监测和病原分析。
Objective To understand the prevalence and characteristics of diarrhea in children hospitalized with diarrhea in Beijing from 2007 to 2008 at a monitoring point of Guangxi Zhuang Autonomous Region. Methods Stool samples from hospitalized children with acute diarrhea under 5 years of age at a monitoring point in Guangxi were collected. The HuCVs were detected by RT-PCR, and some positive samples were sequenced and nucleotide sequences were compared to classify the virus types. Results The average positive rate of HuCVs between 2007 and 2008 was 13.3% (81/607), and the peak of infection was between October and October. Fifty-eight strains of HuCVs were sequenced and analyzed, all belonging to the GII-4 type, and 57 strains were similar to the Europe-2006b strain. Conclusions Guangxi cup-shaped virus diarrhea peak earlier than other regions. GII-4 / 2006b strain is the predominant pandemic strain of HuCVs diarrhea at a monitoring point in Guangxi in 2007-2008. Long-term monitoring and pathogen analysis of infant calicivirus diarrhea in Guangxi should be conducted .