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目的通过观察结核病患者外周血和胸水中IL-8的水平,检测TLR2配体的活性,探讨TLR2在结核病发病中的作用。方法选取肺结核(PTB)患者45例,结核性胸膜炎(TP)患者25例,健康对照(HD)38例,采用ELISA法分别检测血浆和胸水中IL-8含量,并用HEK293-hTLR2细胞株检测血浆和胸水中TLR2配体的活性。结果发现IL-8在结核病患者的胸水中水平明显升高(P<0.001),而且结核病人中的血浆和胸水TLR2配体的活性也高于健康者(P<0.05)。结论结核病患者感染部位TLR2配体活性及IL-8明显升高,对局部炎症细胞的浸润有重要意义,同时可作为结核病诊断的辅助手段。
Objective To observe the level of IL-8 in peripheral blood and pleural effusion of patients with tuberculosis and to detect the activity of TLR2 ligand and to explore the role of TLR2 in the pathogenesis of tuberculosis. Methods 45 cases of tuberculosis (PTB), 25 cases of tuberculous pleurisy (TP) and 38 cases of healthy control (HD) were selected. The content of IL-8 in plasma and pleural effusion were determined by ELISA. And TLR2 ligand activity in pleural effusion. The results showed that the level of IL-8 in pleural effusion of tuberculosis patients was significantly higher (P <0.001), and the activity of TLR2 ligand in plasma and pleural fluid in tuberculosis patients was also higher than that in healthy people (P <0.05). Conclusion The TLR2 ligand activity and IL-8 in the infected area of patients with tuberculosis are significantly increased, which is of great significance to the infiltration of local inflammatory cells and can be used as an adjunct to tuberculosis diagnosis.