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为探讨人肝癌中肿瘤转移抑制基因ｎｍ２３Ｈ１的ｍＲＮＡ表达状况，设计特异性引物，应用逆转录聚合酶链反应（ＲＴＰＣＲ）技术检测分析了２０例人肝癌组织及相应癌旁肝组织中ｎｍ２３Ｈ１基因的ｍＲＮＡ表达。结果：特异性引物能成功扩增ｎｍ２３Ｈ１基因的几乎整个编码序列；２０例肝癌及癌旁肝组织的ＲＴＰＣＲ结果均为阳性，ｎｍ２３Ｈ１基因ｍＲＮＡ未发生表达缺失或较大变异现象。由此表明：本实验建立的ＲＴＰＣＲ方法为进一步定量测定肝癌组织中ｎｍ２３Ｈ１ｍＲＮＡ表达水平奠定了基础。
In order to investigate the expression of nm23-H1 mRNA in human hepatocellular carcinoma, specific primers were designed and 20 cases of human hepatocellular carcinoma and corresponding para-cancer liver tissues were detected by reverse transcriptase polymerase chain reaction (RT-PCR). nm23-H1 mRNA expression. RESULTS: Specific primers could successfully amplify the entire coding sequence of nm23-H1 gene; RT-PCR results were positive in 20 cases of hepatocellular carcinoma and paraneoplastic liver tissue, and no expression or deletion of nm23-H1 gene mRNA was observed. . This indicates that the RT-PCR method established in this experiment lays the foundation for further quantitative determination of nm23-H1 mRNA expression in HCC tissue.