nm23-H1与大肠癌淋巴结转移的相关性研究

• 作 者: 李升平 朱振宇 王辉云 王吉甫 朱全胜 刘锦怀
• 机 构: 中山医科大学,广州市第一人民医院
• 出 处: 中山医科大学学报
• 发表日期: 1998年02期
• 关键词: 大肠癌转移 nm23-H1 淋巴结转移 结肠直肠肿瘤 正常粘膜 基因表达 淋巴转移 生物学指标 组织切片 杂合性缺失

目的：探讨ｎｍ２３Ｈ１与大肠癌淋巴结转移的关系。方法：利用限制性片段长度多态性、Ｓｏｕｔｈｅｒｎｂｌｏｔ和免疫组织化学染色技术（ＬＳＡＢ法），检测８０例大肠癌及正常粘膜ＤＮＡ中ｎｍ２３Ｈ１杂合性缺失（ｎｍ２３Ｈ１ＬＯＨ）和石蜡组织切片中ｎｍ２３Ｈ１蛋白表达。结果：８０例中４５例为杂合子，其中ＬＯＨ１４例，９例有淋巴结转移，３１例无ＬＯＨ者，仅６例有淋巴结转移（Ｐ＜０．０１）；ｎｍ２３Ｈ１蛋白表达阴性２０例中１１例有淋巴结的转移，表达阳性２５例中４例有淋巴结转移（Ｐ＜０．０１）。ｎｍ２３Ｈ１ＬＯＨ和ｎｍ２３＋Ｈ１蛋白表达阴性者在ＤｕｋｅｓＣ／Ｄ期较Ａ、Ｂ期多见（Ｐ＜０．０５）。ＬＯＨ和蛋白表达与年龄、性别、肿瘤大小、部位、组织类型无关。结论：ｎｍ２３Ｈ１ＬＯＨ与大肠癌淋巴结转移呈正相关，蛋白表达与大肠癌淋巴结转移呈负相关；检测大肠癌组织ＤＮＡ中ｎｍ２３Ｈ１ＬＯＨ和组织切片中ｎｍ２３Ｈ１蛋白表达，可能成为预测大肠癌淋巴结转移的生物学指标之一。

Objective: To investigate the relationship between nm23H1 and lymph node metastasis of colorectal cancer. METHODS: Using restriction fragment length polymorphism, Southern blot, and immunohistochemical staining (LSAB) to detect nm23H1 heterozygosity loss (nm23H1LOH) and paraffin sections in 80 cases of colorectal cancer and normal mucosal DNA nm23-H1 protein expression. Results: Of the 80 cases, 45 cases were heterozygous, including 14 cases with LOH, 9 cases with lymph node metastasis, 31 cases without LOH, and 6 cases with lymph node metastasis (P<0.01); nm23-H1 protein expression was negative in 20 cases. 11 cases had lymph node metastasis, and 4 of 25 cases showed lymph node metastasis (P<0.01). The negative expression of nm23H1LOH and nm23+H1 was more common in Dukes C/D than in A and B (P<0.05). LOH and protein expression were independent of age, gender, tumor size, location, and tissue type. Conclusion: nm23H1LOH is positively correlated with lymph node metastasis of colorectal cancer. Protein expression is negatively correlated with lymph node metastasis of colorectal cancer. Detection of nm23H1LOH in colorectal tissue DNA and expression of nm23H1 protein in tissue slices may be a predictor of lymph node metastasis in colorectal cancer. One of the biological indicators.