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目的了解贵阳地区夏秋季感染性腹泻病例中致泻性大肠杆菌的分布状况,为该地区致泻性大肠杆菌的预防控制提供依据。方法收集2010年贵阳地区6家哨点医院的381例感染性腹泻病例的粪便标本,提取标本DNA,采用实时荧光PCR(RT-PCR)方法进行肠致病性大肠杆菌(Enteropathogenic E Coli,EPEC)、肠产毒性大肠杆菌(Enterotoxigenic E coli,ETEC)、肠粘附性大肠杆菌(Enteroadherent E coli,EAEC)、肠侵袭性大肠杆菌(Enteroinvasive E coli,EIEC)、肠出血性大肠杆菌(Enterohemorrhagic E coli,EHEC)的检测,并进行病原统计分析。结果 381例腹泻病例的粪便标本中致泻性大肠杆菌检出率为34.65%,其中检出率最高的是EAEC,为12.07%;其次是EPEC,检出率为9.45%,其中非典型EPEC占75.00%;ETEC检出率为9.19%,其中ETEC-ST占60.00%;EIEC检出率为3.94%;EHEC未检出;两种病原混合感染检出率为5.25%,以EPEC和EAEC混合感染为主,占40.00%。≤5岁组和>5岁组病例病原检出率分别为35.28%和30.91%,差异无统计学意义(P>0.05);≤5岁组病原EAEC检出率较高(P<0.05);>5岁组病原ETEC检出率较高(P<0.05)。男性病例检出率高于女性(P<0.05)。8月、9月检出率相对较高(P<0.001)。结论贵阳地区腹泻病例致泻性大肠杆菌感染以EAEC为主,其次是EPEC和ETEC,未发现有EHEC,8月、9月贵阳地区致泻性大肠杆菌的检出率较高。
Objective To understand the distribution of diarrhea-causing Escherichia coli in infectious diarrhea cases in summer and autumn in Guiyang, and to provide evidence for the prevention and control of diarrheogenic E. coli in this area. Methods A total of 381 cases of infectious diarrhea were collected from 6 sentinel hospitals in Guiyang in 2010, and DNA was extracted from the samples. Enteropathogenic E Coli (EPEC) was obtained by real-time fluorescence PCR (RT-PCR) , Enterotoxigenic E. coli (ETEC), Enteroadherent E. coli (EAEC), Enteroinvasive E coli (EIEC), Enterohemorrhagic E coli , EHEC) detection, and pathogen statistical analysis. Results The detection rate of diarrheogenic E. coli in stool samples from 381 cases of diarrhea was 34.65%. The highest detection rate was 12.07% for EAEC, followed by EPEC, the detection rate was 9.45% 75.00%; the detection rate of ETEC was 9.19%, ETEC-ST accounted for 60.00%; the detection rate of EIEC was 3.94%; EHEC was not detected; the detection rate of mixed pathogens between the two pathogens was 5.25% Mainly, accounting for 40.00%. The detection rate of pathogens in cases of ≤5 years old and> 5 years old was 35.28% and 30.91% respectively, with no significant difference (P> 0.05). The detection rate of EAEC in ≤5 years old group was higher (P <0.05). The detection rate of ETEC in> 5-year-old group was higher (P <0.05). The detection rate of male cases was higher than that of women (P <0.05). The detection rates in August and September were relatively high (P <0.001). Conclusion The diarrhea-causing Escherichia coli in diarrhea cases in Guiyang are predominantly EAEC, followed by EPEC and ETEC, with no EHEC detected. The detection rate of diarrheogenic E. coli in August and September in Guiyang is higher.