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应用单克隆抗体和免疫组化ABC法对53例肺癌的局部免疫状态及β_2微球蛋白(简称β_2 M)表达进行研究。结果表明:①机体对肺癌组织以细胞免疫为主,局部反应主要为T细胞,伴少量巨噬细胞和B细胞。免疫细胞主要分布于肺癌间质,仅少数散在于癌巢中。②肺癌局部T细胞多处于非活化状态。OKT_4/OKT_8比值各例不同,平均为1.24±0.59。③有淋巴结转移的肺癌组,其局部T细胞和活化T细胞数低于无转移组,而巨噬细胞则是转移组高于无转移组。鳞癌中活化T细胞和巨噬细胞高于未分化癌。以上差别均有显著或极显著意义。④肺癌分化好者β_2 M表达强,分化差者表达弱。β_2 M的表达与全T,辅助T和活化T细胞的浸润呈正相关。本文认为肺癌组织中免疫细胞的浸润及其活性状态可作估计预后的指标之一。β_2 M表达可做肺癌分级的参考指标。
The local immune status and the expression of β 2 microglobulin (abbreviated as β 2 M) in 53 cases of lung cancer were studied using monoclonal antibodies and immunohistochemical ABC method. The results showed that: 1 The body’s lung cancer tissue is mainly cell-based, and the local response is mainly T-cells, with a few macrophages and B-cells. The immune cells are mainly distributed in the lung cancer stroma, and only a few are scattered in the cancer nest. 2 Most local T cells in lung cancer are inactive. The ratio of OKT_4/OKT_8 varies from case to case, with an average of 1.24±0.59. 3 In the lung cancer group with lymph node metastasis, the number of local T cells and activated T cells was lower than that of non-metastasis group, while macrophages were higher in the metastatic group than in the no metastasis group. Activated T cells and macrophages in squamous cell carcinoma are higher than those in undifferentiated carcinoma. The above differences are significant or extremely significant. 4 The expression of β 2 M in lung cancer patients with good differentiation was strong, and poor expression in poorly differentiated patients. The expression of β 2 M was positively correlated with all T, T helper and activated T cell infiltration. In this paper, the infiltration and activity status of immune cells in lung cancer can be used as one of the indexes to estimate the prognosis. Β_2 M expression can be used as a reference index for lung cancer grading.