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为了研究14种冰草(Agropyron cristatum)在20%聚乙二醇(PEG)模拟干旱胁迫下同工酶的变化,试验采用垂直聚丙烯酰胺凝胶电泳方法对冰草叶片过氧化物同工酶(POD)、过氧化氢同工酶(CAT)和酯酶(EST)同工酶活性的变化进行分析。结果表明:PEG胁迫下14种冰草POD、CAT、EST同工酶酶带数和活性、带级与对照各不相同,POD同工酶谱带共出现181条,最多的有9条,最少的有4条,出现的酶带类型为17种;CAT同工酶酶谱共出现185条,最多的有10条,最少的有4条,出现的酶带类型为15种;EST同工酶酶谱共出现176条,最多的有10条,最少的有4条,出现的酶带类型为16种;3种同工酶的变化与冰草对PEG胁迫的抗性不同有关,蒙农杂种冰草的抗旱性比较强,而中间冰草的抗旱性比较弱。
In order to study the changes of isozymes of fourteen Agropyron cristatum strains under the simulated drought stress of 20% polyethylene glycol (PEG), the vertical polyacrylamide gel electrophoresis (POD), hydrogen peroxide (CAT) and esterase (EST) isozyme activities were analyzed. The results showed that the enzyme bands and activities of POD, CAT and EST isozymes of 14 species were respectively different under PEG stress, with bands of POD isozymes and bands of 181 isolates, up to 9 bands and the least There were 4 bands and 17 bands appeared. CAT isozyme bands appeared in 185 bands, up to 10 bands, at least 4 bands and 15 bands. EST isozymes The total number of zymograms was 176, of which 10 were the highest, 4 were the least, and the types of bands appeared were 16 species. The changes of 3 isoenzymes were related to the resistance of Agropyron crassipes to PEG stress. Ice grass drought resistance is relatively strong, and the mid-ice grass drought resistance is relatively weak.