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作者以病毒感染培养细胞沉淀的石蜡包埋块为材料提取RNA,并经逆转录-多聚酶链反应(RT-PCR)扩增检测病毒RNA,比较不同的固定液及提取方法,选择最佳方案。以不同长度(82～507bp)片段的肌动蛋白mR-NA作为抽提物中细胞RNA的对照。对实验标本石蜡包埋前的处理分别是:福马林(6h、24h或5个月),多聚甲醛(30min),Carnoy氏(2h),柠檬酸缓冲丙酮(5min)以及B-5固定液(2h),结果表明福马林固定的标本能适用于PCR,但丙酮固定的
In this study, we extracted RNA from virus-infected cultured paraffin blocks and detected the viral RNA by reverse transcription-polymerase chain reaction (RT-PCR). Compare the different fixatives and extraction methods and choose the best protocol. The actin mR-NA of different length (82 ~ 507bp) fragment was used as the control of cell RNA in the extract. Paraffin embedded in the experimental specimens were treated with formalin (6h, 24h or 5 months), paraformaldehyde (30min), Carnoy’s (2h), citrate buffered acetone (5min) and B-5 Liquid (2h), the results show that the fixed specimen of fmalin can be applied to PCR, but the acetone fixed