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目的探讨自发性细胞凋亡及其相关基因bcl-2和bax与鼻咽癌放射敏感性的关系。方法采用TdT酶介导的生物素化dUTP缺口末端标记技术(terminal oxynucleotidyl transferase medi-ated dUTP biotin nick end labeling,TUNEL)和免疫组化法,分别检测51例放疗前的鼻咽癌活检组织中自发性凋亡指数(spontaneous apoptotic index,SAI)和bcl-2、bax基因的表达。结果鼻咽癌活检组织均检测到细胞凋亡,SAI平均为31.19±33.83;SAI与bcl-2和bax表达均无明显相关性,但与bcl-2/bax比率呈负相关(P<0.05);SAI与鼻咽癌放射敏感性明显相关,完全消退组比残留组SAI高(P<0.05);bcl-2的表达与鼻咽癌放射敏感性呈负相关(P<0.05),而bax的表达与鼻咽癌放射敏感性无关(P>0.05);bcl-2/bax的比率与鼻咽癌放射敏感性呈负相关(P<0.05)。结论SAI、bcl-2和bcl-2/bax的比率是反映鼻咽癌放射敏感性和预测鼻咽癌放疗效果的重要指标,而bax不能单独作为判断鼻咽癌放射敏感性的指标。
Objective To investigate the relationship between spontaneous apoptosis and related gene bcl-2 and bax and radiosensitivity of nasopharyngeal carcinoma. Methods TdT-mediated terminal oxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) and immunohistochemistry were used to detect the spontaneous Spontaneous apoptotic index (SAI) and bcl-2, bax gene expression. Results Apoptosis was detected in all the biopsy specimens of nasopharyngeal carcinoma with an average SAI of 31.19 ± 33.83. There was no significant correlation between SAI expression and bcl-2 and bax expression but negative correlation with bcl-2 / bax (P <0.05) SAI was significantly correlated with radiosensitivity of nasopharyngeal carcinoma (P <0.05); the expression of bcl-2 was negatively correlated with radiosensitivity of nasopharyngeal carcinoma (P <0.05); while the expression of bax The expression of bcl-2 / bax was negatively correlated with radiosensitivity of nasopharyngeal carcinoma (P <0.05). Conclusions The ratio of SAI, bcl-2 and bcl-2 / bax is an important index to reflect the radiosensitivity of nasopharyngeal carcinoma and predict the radiotherapy efficacy of nasopharyngeal carcinoma. However, bax can not be used alone as an index to determine the radiosensitivity of nasopharyngeal carcinoma.