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目的研究孕龄与人巨细胞病毒宫内感染的垂直传播率之间的关系。方法分别应用ELISA方法和PCR方法检测273例不同孕龄的孕妇外周血标本中的HCMV-IgM和HCMV-DNA,新生儿血样或胚胎组织用PCR方法检测HCMV-DNA以诊断先天性HCMV感染。结果孕早期妇女外周血中的HCMV-IgM和HCMV-DNA的阳性率均低于孕中期和孕晚期妇女,差异有显著性(P<0.005)。孕中期和孕晚期妇女宫内感染的垂直传播率显著高于孕早期妇女,差异有统计学意义(P<0.001)。结论孕中期和孕晚期妇女发生宫内感染的几率和垂直传播率均显著大于孕早期妇女,应加强对孕中期和孕晚期HCMV感染孕妇的监测和治疗。
Objective To study the relationship between gestational age and the vertical transmission rate of human cytomegalovirus intrauterine infection. Methods HCMV-IgM and HCMV-DNA in 273 pregnant women with different gestational ages were detected by ELISA and PCR respectively. HCMV-DNA was detected by PCR in neonatal blood samples or embryos to diagnose congenital HCMV infection. Results The positive rates of HCMV-IgM and HCMV-DNA in peripheral blood of pregnant women were lower than those of the third trimester and the third trimester of pregnancy. The difference was significant (P <0.005). The vertical transmission rate of intrauterine infection in the second trimester and third trimester women was significantly higher than that in the first trimester, the difference was statistically significant (P <0.001). Conclusion The incidence of intrauterine infection and vertical transmission in pregnant women in the second and third trimester were significantly higher than those in the first trimester. The monitoring and treatment of pregnant women with HCMV infection during the second trimester and third trimester should be strengthened.