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目的:了解江苏省2012年柯萨奇病毒A组16型(CA16)分离株VP1区基因特征。方法:选取江苏省2012年14株CA16病毒分离株,用1对特异性引物进行VP1区核苷酸序列扩增,对扩增产物进行测序,利用生物信息学软件对序列进行分析,结果与CA16参考株序列进行同源性比较并构建基因进化树。结果:14株CA16分离株VP1区核苷酸和氨基酸同源性分别为90.5%~100.0%和98.7%~100.0%,与CA16国际标准株G10的核苷酸和氨基酸同源性分别为75.5%~76.7%和91.6%~92.3%。江苏省2012年的CA16分离株全部属于B1基因亚型,同时包含B1a和B1b两条进化分支。结论:江苏省2012年有CA16病毒的B1a与B1b两种进化分支共同存在与循环,且呈现以B1b基因亚型为优势型别、B1a基因亚型为辅助型别的传播模式;无论是优势型别还是辅助型别,均各自呈现密切的亲缘关系。
Objective: To understand the gene characteristics of VP1 region of Coxsackievirus A group 16 (CA16) isolates in Jiangsu Province in 2012. Methods: A total of 14 CA16 isolates were selected from Jiangsu Province in 2012, and a pair of specific primers were used to amplify the nucleotide sequence of VP1 region. The amplified products were sequenced and analyzed by bioinformatics software. The results were compared with those of CA16 Homologous comparison with reference strain sequence and construction of phylogenetic tree. Results: The nucleotide and amino acid homologies of VP1 of 14 CA16 isolates were 90.5% -100.0% and 98.7% -100.0%, respectively. The nucleotide and amino acid homologies with CA16 international standard strain G10 were 75.5% ~ 76.7% and 91.6% ~ 92.3% respectively. All the CA16 isolates in Jiangsu Province in 2012 belonged to the B1 gene subtype and contained two evolutionary branches, B1a and B1b. CONCLUSION: The two evolutionary branches B1a and B1b of CA16 virus coexisted and circulated in Jiangsu province in 2012, and presented the transmission mode with B1b genotype as the predominant type and B1a genotype as the auxiliary type. No matter the predominant type Do not or auxiliary type, all showed close genetic relationship.