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鼠源抗体对人的异源免疫原性问题直接导致了人源抗体技术的出现,经过十几年的研究,该技术日臻成熟.嵌合和人源化抗体的研究巳经取得了很大的成功,巳有产品应用于临床,但在实践中有其局限性.噬菌体抗体库体系的创建,用细菌克隆代替B细胞克隆,是人源抗体技术又一次突破性进展.噬菌体抗体操作简便,可以从中筛选出包括中和抗体、毒素抗体、多糖抗体、自身抗体、抗全细胞抗体在内的各种高亲和力抗体.转基因动物抗体技术于叨年代初出现,技术还不大成熟,目前人们只能将一部分人的Ig全套胚系基因转移到动物体内,但发展前景不可限量.
The problem of heterologous immunogenicity of murine antibody to human directly leads to the emergence of human antibody technology, which has become more and more mature after more than 10 years of research. The studies on chimeric and humanized antibodies have made great achievements Success, have been applied to clinical products, but in practice has its limitations. The establishment of phage antibody library system, the use of bacterial cloning instead of B cell clones, human antibody technology is another breakthrough. Easy to operate phage antibodies, you can From which screening of neutralizing antibodies, toxin antibodies, polysaccharide antibodies, autoantibodies, anti-whole cell antibodies, including a variety of high-affinity antibodies.Transgenic antibody antibody technology in the early nineties, the technology is not yet mature, at present only one Some of the human Ig full set of germline genes transferred to animals, but the development of an unlimited number of prospects.