广西肝癌组织中P~（53）蛋白和乙肝病毒免疫学标志物的检测

• 作 者: 罗丹 刘启福 苏建家 覃国忠 覃柳亮 段小娴
• 机 构: 广西肿瘤研究所
• 出 处: 广西医科大学学报
• 发表日期: 1996年04期
• 关键词: 肝癌发生 乙肝病毒 癌旁肝组织 肝细胞癌 人肝癌 免疫组化法 基因突变 乙型肝炎病毒 阳性病例 免疫学

用免疫组化法测定广西２９例人肝癌及其癌旁组织中的Ｐ５３蛋白、ＨＢｓＡｇ和ＨＢｘＡｇ。４４．８％肝癌病例为Ｐ５３蛋白阳性，其癌内及癌旁Ｐ５３蛋白的检出率分别为２７．６％和２８．６％。２１％的肝癌内及８５．７％的癌旁肝组织中可见到ＨＢｓＡｇ。３１％的癌内及７５％的非癌肝组织呈ＨＢｘＡｇ阳性。Ｐ５３蛋白阳性的１３例病例有１１例（８４．６％）可同时检测出ＨＢｓＡｇ和ＨＢｘＡｇ。结果显示：广西肝癌与Ｐ５３基因突变、乙型肝炎病毒（ＨＢＶ）感染关系密切。

The immunohistochemistry method was used to determine P53 protein, HBsAg and HBxAg in 29 cases of human hepatocellular carcinoma and its adjacent tissues. 44.8% of liver cancer cases were positive for P53 protein, and the detection rates of cancer and paracancerous P53 protein were 27.6% and 28.6%, respectively. HBsAg was found in 21% of liver cancers and 85.7% of cancerous liver tissues. 31% of cancers and 75% of non-cancerous liver tissues were positive for HBxAg. In 13 cases with positive P53 protein, 11 cases (84.6%) could simultaneously detect HBsAg and HBxAg. The results showed that liver cancer in Guangxi is closely related to the mutation of P53 gene and hepatitis B virus (HBV) infection.