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目的 :研究不同浓度丙泊酚 (异丙酚 )对内毒素刺激小鼠腹腔巨噬细胞TNF α ,IL 6 ,IL 8mRNA表达的影响。方法 :分离巯基乙酸盐诱导的小鼠腹腔巨噬细胞 ,随机分为 7组。A组 :阴性对照组 ;B组 :阳性对照组 ,脂多糖(LPS) 10ng/ml孵育细胞 ;C组 :单用丙泊酚组 ,5 0 0 μg/ml孵育细胞 ;D ,E ,F ,G组 :丙泊酚 +LPS组 ,不同浓度丙泊酚(0 .5 ,5 ,5 0 ,5 0 0 μg/ml)孵育 2h后加入LPS 10ng/ml继续孵育 1～ 4h。用RT PCR法检测巨噬细胞TNF α ,IL 6 ,IL 8mRNA的水平。结果 :在内毒素诱导下 ,腹腔巨噬细胞TNF α ,IL 6 ,IL 8mRNA的水平均显著增高 ;丙泊酚对LPS刺激后TNF α ,IL 6mRNA表达水平的增高有抑制作用 ,并呈浓度依赖性 ;但对LPS刺激下IL 8mRNA表达水平增高的影响无统计学意义。结论 :丙泊酚能在转录水平抑制内毒素刺激下小鼠腹腔巨噬细胞促炎性细胞因子的产生。
Objective: To investigate the effects of different concentrations of propofol on the expression of TNFα, IL-6 and IL-8 in mouse peritoneal macrophages stimulated by endotoxin. Methods: Thioglycollate-induced murine peritoneal macrophages were isolated and randomly divided into 7 groups. Group A: negative control group; Group B: positive control group, lipopolysaccharide (LPS) incubated with 10ng / ml; Group C: propofol alone group, incubated cells at 500 μg / Group G: Propofol + LPS group, different concentrations of propofol (0.5, 5, 500, 500 μg / ml) were incubated for 2h and then added LPS 10ng / ml and incubated for 1 ~ 4h. The levels of TNFα, IL 6 and IL 8 mRNA in macrophages were detected by RT-PCR. Results: The levels of TNFα, IL-6 and IL-8 mRNA in peritoneal macrophages induced by endotoxin were significantly increased. Propofol inhibited the expression of TNFα and IL-6 mRNA in LPS-stimulated cells in a concentration-dependent manner However, the effect of LPS stimulation on IL 8 mRNA expression was not statistically significant. Conclusion: Propofol can inhibit the production of proinflammatory cytokines in mouse peritoneal macrophages stimulated by endotoxin at the transcriptional level.