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为给评价声称具有提高免疫的能力的含乙醇保健食品提供依据 ,观察不同剂量乙醇对小鼠免疫系统的影响。采用 17～ 2 0g的雄性BalB c小鼠 90只 ,随机分为 6组 ,每组 15只 ,分别以 0、0 6 7、1 33、3 33、6 0 8、8 0 0g kgBW 乙醇每天一次灌胃给予 ,灌胃量为 2 0mL kgBW,连续灌胃 30d后 ,分别测定细胞免疫、体液免疫、单核巨噬细胞及NK细胞活性等 7项指标。结果表明 :(1)乙醇可以导致小鼠的免疫系统损伤。 (2 ) 8 0 0g kgBW(5 0 % )剂量组在一次灌胃后 2 0min内全部进入昏睡状态 ,2 4h内全部死亡。 (3) 6 0 8g kgBW(38% )组小鼠死亡率为 82 2 %。存活小鼠碳粒廓清能力低下 ,至 30d时仍未恢复。 (4 ) 1 33g kgBW(8% )组腹腔巨噬细胞吞噬鸡红细胞能力 (吞噬率 )降低 ,但对鸡红细胞吞噬指数无显著性影响。 (5 ) 0 6 7g kgBW(4 % )组NK细胞活性降低。 (6 ) 3 33g kgBW(2 0 % )组小鼠的 7项免疫指标均无显著性影响。 (7)以酒精为载体的保健食品在进行免疫调节作用评价实验时 ,小鼠灌胃液中酒精的浓度不宜超过 2 0 %。
In order to provide a basis for evaluating the health food containing ethanol which is claimed to have immunity enhancement, the effects of different doses of ethanol on the immune system of mice were observed. Ninety Balb c mice (17-20 g) were randomly divided into 6 groups (15 rats in each group) with 0, 067, 33, 33, 80, 80 g kg BW ethanol once a day Gavage, gavage of 20mL kgBW, continuous gavage 30d, were measured cellular immunity, humoral immunity, mononuclear macrophage and NK cell activity and other seven indicators. The results showed that: (1) ethanol can cause damage to the immune system in mice. (2) In the dose of 80 kg kgBW (50%), the rats all entered the lethargic state within 20 min after intragastric administration, and all died within 24 h. (3) The mortality rate of mice in the group of 688 kgBW (38%) was 82.2%. Survival mice carbon clearance is low, to 30d still did not recover. (4) The capacity (phagocytosis rate) of phagocytosis of chicken erythrocytes by 1 33 g kgBW (8%) group decreased, but had no significant effect on phagocytic index of chicken erythrocytes. (5) NK cell activity decreased in 0 6 7g kgBW (4%) group. (6) There were no significant effects of the seven immune indexes in 3 33 g kgBW (20%) mice. (7) Alcohol-based health food In the evaluation of immunomodulatory experiments, the concentration of alcohol in the gastric juice should not exceed 20%.