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聚合酶链反应(Polymerase Chain Reaction),简称PCR,是新近建立的一种体外扩增特异性DNA的技术,具有快速、简便、敏感度高、特异性强等优点。近年来,国外应用PCR技术对乙型肝炎病毒(HBV)感染进行了大量的研究,并显示广泛的应用前景,国内处于开始研究阶段。现就PCR技术在HBV检测中的应用作一概述。一、发展诊断HBV感染的新方法现已证实,每毫升污染10~2个Dane颗粒的血清即能使黑猩猩发生HBV感染。过去采用的分子杂交法仅可检出0.1Pg/ml的HBVDNA,相当于10~5～10~6Dane颗粒,限制了对HBV感染的诊断。PCR技术可检出低至0.4f g/ml(130 Dane颗粒/ml)水平的
Polymerase Chain Reaction, referred to as PCR, is a newly established technology for amplifying specific DNA in vitro. It has the advantages of fast, simple, high sensitivity and specificity. In recent years, foreign applications of PCR technology for hepatitis B virus (HBV) infection carried out a large number of studies, and shows a wide range of applications, the country is at the beginning of the research phase. Now on PCR technology in the detection of HBV make an overview. First, the development of a new method of diagnosis of HBV infection It has been confirmed that per ml contaminated with 10 ~ 2 Dane particles of serum that chimpanzees can make HBV infection. The molecular hybridization method used in the past only detected 0.1Pg / ml of HBVDNA, equivalent to 10 ~ 5 ~ 10 ~ 6Dane particles, limiting the diagnosis of HBV infection. PCR technology detects levels as low as 0.4 fg / ml (130 Dane particles / ml)