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目的建立同时测定清胰利胆颗粒中绿原酸和芍药苷含量的方法。方法采用HPLC法。色谱柱:Kromasil C18柱(250 mm×4.6 mm,5μm),流动相:乙腈(A)-体积分数为0.1%的磷酸水溶液(B),梯度洗脱程序:0 min-10%(φ)A、9 min-13%(φ)A、13 min-13%(φ)A、14 min-16%(φ)A、22min-17%(φ)A、30 min-17%(φ)A、40 min,40%(φ)A,流速:1.0 mL.min-1,检测波长:327 nm(0～14 min)和230 nm(14～40 min)。结果绿原酸和芍药苷质量浓度分别在5.2～51.6 mg.L-1(r=0.999 6,n=6)、9.9～99.2 mg.L-1(r=0.999 8,n=6)内与峰面积线性关系良好,平均回收率分别为100.5%(RSD=1.9%,n=6)、100.2%(RSD=2.2%,n=6)。结论该方法可作为清胰利胆颗粒质量控制方法之一。
Objective To establish a method for the simultaneous determination of chlorogenic acid and paeoniflorin in Qingyao Lidan Granules. Methods HPLC method. Column: Kromasil C18 column (250 mm × 4.6 mm, 5 μm) with mobile phase of acetonitrile (A) - 0.1% phosphoric acid aqueous solution (B), gradient elution program: 0 min-10% , 13 min-13% (φ) A, 14 min-16% (φ) A, 22 min -17% (φ) A, 30 min -17% 40 min (φ) A, flow rate 1.0 mL.min-1, detection wavelength 327 nm (0-14 min) and 230 nm (14-40 min). Results The concentrations of chlorogenic acid and paeoniflorin were respectively in the range of 5.2-51.6 mg.L-1 (r = 0.999 6, n = 6) and 9.9-99.2 mg.L-1 (r = 0.999 8, n = 6) The peak area was linear and the average recoveries were 100.5% (RSD = 1.9%, n = 6) and 100.2% (RSD = 2.2%, n = 6). Conclusion This method can be used as one of the quality control methods of Qingyi Liguan granule.