论文部分内容阅读
目的为探讨喉癌与人乳头瘤病毒（ＨＰＶ）感染的关系和ＨＰＶ在喉癌中基因组型的分布与表达。方法应用聚合酶链反应技术（ＰＣＲ）制备非放射性探针标记物－地高辛标记ＨＰＶ共有引物探针，对１４６例喉不同病变的新鲜组织标本（喉癌６８例，喉其它病变４８例，正常喉组织３０例），进行ＨＰＶ６，１１，１６，１８，３１，３３，３５，４２，５８共９型ＨＰＶＤＮＡ感染的检测；阳性者用多重引物ＰＣＲ方法分型。结果喉癌ＨＰＶ感染阳性率４５．６％（３１／６８），喉癌颈转移淋巴结组织阳性率２０．０％（３／１５），喉癌前病变阳性率１１．８％（２／１７），声带息肉阳性率６．３％（１／１６），１５例癌旁及１５例癌周正常喉组织均为ＨＰＶＤＮＡ阴性。ＨＰＶＤＮＡ型别分布在喉癌中以ＨＰＶ１６、１８型为主，喉良性病变中以ＨＰＶ６、１１型为主。结论喉癌发生与ＨＰＶ感染有关。
Objective To investigate the relationship between laryngeal cancer and human papillomavirus (HPV) infection and the genomic distribution and expression of HPV in laryngeal cancer. Methods A total of 146 specimens of larynx with different pathological changes (68 cases of laryngeal cancer, 48 cases of other laryngeal lesions, 30 cases of normal laryngeal tissue), HPV6,11,16,18,31,33,35,42,58 a total of 9 detection of HPVDNA infection; positive with multiple primer PCR typing. Results The positive rate of HPV infection in laryngeal carcinoma was 45.6% (31/68), the positive rate of cervical lymph node metastasis in laryngeal carcinoma was 20.0% (3/15) and the positive rate of laryngeal precancerous lesions was 11.8% (2/17) , The positive rate of vocal cord polyps 6.3% (1/16), 15 cases of adjacent cancer and 15 cases of normal laryngeal cancer were HPVDNA negative. HPVDNA type distribution in laryngeal cancer mainly HPV16,18, benign laryngeal lesions in the HPV6,11 type. Conclusion The occurrence of laryngeal carcinoma is related to HPV infection.