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目的探讨肿瘤坏死因子α(TNF-α)对血管内皮祖细胞(EPCs)产生E-选择素和内皮型一氧化氮合酶(eNOS)活力的影响及不同浓度辛伐他汀的干预作用。方法体外培养与鉴定兔骨髓源EPCs,用不同浓度TNF-α(1、10、25、50 ng/ml)刺激24 h;50 ng/ml TNF-α刺激细胞0.5 h后用不同浓度辛伐他汀(0、0.1、1.0、10μmol/L)干预24 h,收集上清;用ELISA法检测可溶性E-选择素的浓度,用化学比色法测定eNOS活力。结果在TNF-α刺激下EPCs产生E-选择素增加,同时eNOS活力降低(P<0.05),变化的量和TNF-α的浓度呈相关性;加入辛伐他汀后E-选择素明显下降,eNOS活力与辛伐他汀呈明显的浓度依赖性增大(P<0.05)。结论辛伐他汀对于炎症损伤后的EPCs具有保护作用。
Objective To investigate the effects of tumor necrosis factor-α (TNF-α) on the production of E-selectin and endothelial nitric oxide synthase (eNOS) in endothelial progenitor cells (EPCs) and the effects of different concentrations of simvastatin. Methods EPCs were cultured and identified in vitro and stimulated with different concentrations of TNF-α (1, 10, 25 and 50 ng / ml) for 24 h. After stimulation with 50 ng / ml TNF-α for 0.5 h, (0, 0.1, 1.0 and 10 μmol / L) for 24 h, and the supernatant was collected. The concentration of soluble E-selectin was detected by ELISA and the eNOS activity was measured by chemical colorimetry. Results E-selectin increased and eNOS activity decreased (P <0.05). The amount of EPCs was correlated with the concentration of TNF-α. E-selectin was significantly decreased after adding simvastatin, ENOS activity and simvastatin showed a significant concentration-dependent increase (P <0.05). Conclusion Simvastatin has a protective effect on EPCs after inflammatory injury.