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探讨CDH13基因两个单核苷酸多肽性(SNP)位点(rs11646213和rs7195409)与非小细胞肺癌(NSCLC)发生发展的关系。选取2015年6月~2016年6月我院收治的NSCLC患者100例作为观察组,选取同期健康体检人群100例作为对照组。采用实时荧光定量PCR法检测两组患者的CDH13基因启动子区域两个SNP位点(rs11646213,rs7195409);分析两个SNP位点在两组中的分布特征。观察组SNP位点rs11646213等位基因频率和基因型频率与对照组比较差异具有统计学意义(p<0.05),观察组A等位基因频率明显低于对照组(76.2%vs 90.4%),差异具有统计学意义(p<0.05);SNP位点rs7195409等位基因频率和基因型频率在临床Ⅰ+Ⅱ期和Ⅲ+Ⅳ期患者间比较差异具有统计学意义(p<0.05),Ⅰ+Ⅱ期患者G等位基因频率明显高于Ⅲ+Ⅳ期患者(25.8%vs 12.4%),差异具有统计学意义(p<0.05)。CDH13基因SNP位点rs11646213的A等位基因缺失可能与NSCLC发生启动有关,SNP位点rs7195409的G等位基因缺失可能与NSCLC的病情发展有关。
To investigate the relationship between the two single nucleotide polymorphisms (SNPs) of CDH13 gene (rs11646213 and rs7195409) and the development of non-small cell lung cancer (NSCLC). A total of 100 NSCLC patients admitted to our hospital from June 2015 to June 2016 were selected as the observation group and 100 healthy people during the same period as the control group. The two SNPs (rs11646213, rs7195409) in CDH13 gene promoter region of two groups were detected by real-time fluorescence quantitative PCR. The distribution characteristics of the two SNPs in both groups were analyzed. The SNP rs11646213 allele frequency and genotype frequency in the observation group were significantly different from those in the control group (p <0.05). The frequency of allele A in the observation group was significantly lower than that of the control group (76.2% vs 90.4%), and the difference (P <0.05). The SNP rs7195409 allele frequency and genotype frequency were significantly different between patients with stage Ⅰ + Ⅱ and stage Ⅲ + Ⅳ (p <0.05), with stage Ⅰ + Ⅱ The frequency of G allele was significantly higher in patients with stage Ⅲ + Ⅳ (25.8% vs 12.4%), the difference was statistically significant (p <0.05). The deletion of the A allele of SNP rs11646213 in CDH13 gene may be related to the initiation of NSCLC. The deletion of G allele at SNP rs7195409 may be related to the progression of NSCLC.