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从七十年代利用花药培养技术获得单倍体植株以来,单倍体植物在育种应用上的潜力日益显示出来。但是这种潜力只有使单倍体植物加倍成为纯合二倍体才有可能发挥。因此,对于单倍体植物的染色体加倍,许多植物育种工作者进行过多方面的试验研究,提出过多种加倍方法,其中秋水仙碱法应用最为广泛,但加倍成功率并不理想,植株死亡率也较高。近年来有人用秋水仙碱和DMSO结合处理单倍体植株,加倍效果显著提高。就小麦来说,最高成功率有达9.0％
From the seventies using anther culture technology to obtain haploid plants haploid plants in breeding potential has become increasingly evident. But this potential is only possible by doubling haploid plants into homozygous diploids. Therefore, for haploid plants, chromosome doubling, many plant breeders conducted many aspects of experimental study, put forward a variety of doubling methods, including colchicine method is the most widely used, but the double success rate is not ideal, the plant died Rate is higher. In recent years, colchicine and DMSO were used in combination with haploid plants, doubling the effect was significantly improved. For wheat, the highest success rate is 9.0%