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目的：观察ＤＮＡ与骨骼肌肌质网蛋白结合后对肌质网功能的影响。方法：应用差速离心分离大鼠骨骼肌肌质网，并用４５ＣａＣｌ２作为示踪剂，观察质粒ＤＮＡｐｃＤＮＡ３和ｐｃＤＮＡ３／ＡＮＦ对肌质网钙转运功能的影响。结果：两种质粒均可明显增加肌质网Ｃａ２＋摄取，呈明显的量效关系，并且也可促进肌质网钙释放。结论：外源质粒ＤＮＡ可影响肌质网钙转运功能。
Objective: To observe the effect of DNA and sarcoplasmic reticulum protein binding on sarcoplasmic reticulum function. Methods: Skeletal muscle sarcoplasmic reticulum was isolated from rat by differential centrifugation and the effect of plasmid DNA pcDNA3 and pcDNA3 / ANF on calcium transport in sarcoplasmic reticulum was observed with 45 CaCl2 as tracer. Results: Both plasmids could significantly increase Ca2 + uptake in sarcoplasmic reticulum, showing a significant dose-effect relationship, and also promoted calcium release from sarcoplasmic reticulum. Conclusion: Exogenous plasmid DNA can affect sarcoplasmic reticulum calcium transport function.