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以感染内生真菌的人工禾草高羊茅为研究对象,通过比较不同CO2浓度和氮素处理下,感染内生真菌和不染菌植物在种子萌发和营养生长等方面的差异,探讨感染内生真菌的高羊茅对CO2升高的响应.试验结果表明,(1)内生真菌感染影响了宿主种子萌发对CO2浓度升高的响应,CO2浓度升高使得内生真菌感染促进了宿主植株的胚芽和胚根的伸长;(2)CO2浓度升高使高羊茅地下生物量显著增加,只是内生真菌感染植株的地下生物量显著低于不染菌植株;CO2浓度升高使不染菌植株地上部分氮含量显著降低,而对染菌植物无显著影响;(3)内生真菌对宿主响应CO2的影响与氮素供应水平有关,在高浓度CO2和氮素供应下内生真菌感染显著降低了宿主植物的分蘖数,而在高浓度CO2和缺氮供应下,染菌植株的可溶性糖含量显著高于不染菌植株;(4)内生真菌对宿主响应CO2的影响与氮素供应形态有关,在高浓度CO2和硝态氮供应下,内生真菌感染显著降低了宿主植物的地上生物量,而在铵态氮供应下,染菌植株的总游离氨基酸含量显著高于不染菌植株.
In order to study the difference of endophyte-infected artificial grasses (Festuca arundinacea) from the grafting of endophytic fungi (Festuca arundinacea), the differences in endogenous and endophyte-infected endophytic fungi The results showed that: (1) Endophytic fungus infection affected the host seed germination response to elevated CO2 concentration, and the increase of CO2 concentration caused the endophyte infection to promote the growth of host plants (2) The CO2 concentration increased the underground biomass of tall fescue significantly, but the biomass of endophyte infected plants was significantly lower than that of non-infected plants. The increase of CO2 concentration did not result in the increase of CO2 concentration (3) The effect of endophytic fungi on host response to CO2 was related to the nitrogen supply level. Under the condition of high concentration of CO2 and supply of nitrogen, endophytic fungi Infected significantly reduced the tiller number of the host plants, while the soluble sugar content of the infected plants was significantly higher than that of the non-infected plants under high concentration of CO2 and nitrogen deficiency. (4) The effect of endophytic fungi on host response to CO2 was similar to that of nitrogen On the supply of forms, in Endophytic fungus infection significantly reduced the aboveground biomass of host plants under high concentration of CO2 and nitrate nitrogen supply, but under ammonium nitrogen supply, the total free amino acids content of infected plants was significantly higher than that of non-infected plants.