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目的:建立食品中金黄色葡萄球菌快速、简便、准确、高效的检测方法。方法:以金黄色葡萄球菌FemB基因中保守序列为目标,设计并合成引物和Taqman探针,构建重组质粒作为标准阳性模板,建立荧光定量检测体系,并考察该方法的灵敏性、特异性和重复性。结果:该法的灵敏度60拷贝/反应体系,能特异区分金黄色葡萄球菌与其它类型的细菌,同时,具有良好的重复性。结论:使用Taqman探针法荧光定量PCR技术能够对金黄色葡萄球菌进行快速准确地定量检测。
Objective: To establish a rapid, simple, accurate and efficient detection method of Staphylococcus aureus in food. Methods: Aiming at the conserved sequence in FemB gene of Staphylococcus aureus, primers and Taqman probes were designed and synthesized. The recombinant plasmid was used as a standard template to establish a fluorescence quantitative detection system. The sensitivity, specificity and repeatability of this method were also investigated Sex. Results: The sensitivity of 60 copies / reaction system, can be distinguished specifically Staphylococcus aureus and other types of bacteria, at the same time, with good repeatability. Conclusion: Taqman probe real-time quantitative PCR can detect Staphylococcus aureus rapidly and accurately.