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目的检测血管内皮生长因子(VEGF)在体外受精-胚胎移植(IVF-ET)超促排卵周期中不孕妇女血清及卵泡液中的水平。方法收集IVF治疗的187个因输卵管因素年龄24～38岁的不孕妇女在促性腺激素(Gn)注射日、人绒毛膜促性腺激素(HCG)注射日的血清和卵泡液,用酶联免疫方法测定VEGF在血清和卵泡液中的水平。结果 HCG日血清VEGF的水平明显高于Gn启动日(135.36±1.87)ng/L与(70.34±1.33)ng/L(P<0.01);随着年龄的增长卵泡液VEGF的水平呈上升趋势(r=0.493,P<0.01)两者呈正相关;获卵数≥20个患者单个卵泡中VEGF的水平低于获卵数≤5个的患者(1299.63±43.70)ng/L与(2460.23±548.45)ng/L,但差异无统计学意义(P>0.05)。结论随着促排卵过程中卵泡的发育,血清中VEGF的水平明显增多;随着年龄的增加VEGF在卵泡液中的水平明显升高。
Objective To detect the levels of vascular endothelial growth factor (VEGF) in the serum and follicular fluid of infertile women during IVF-ET. Methods Serum and follicular fluid of 187 infertile women aged 24-38 years due to tubal factors treated with IVF were collected on the day of Gn injection and human chorionic gonadotropin (HCG) injection. Enzyme-linked immunosorbent assay Methods VEGF levels in serum and follicular fluid were measured. Results The level of serum VEGF in HCG day was significantly higher than that of Gn (135.36 ± 1.87) ng / L and (70.34 ± 1.33) ng / L (P <0.01), and the level of VEGF in follicular fluid increased with age (1299.63 ± 43.70) ng / L and (2460.23 ± 548.45) patients, respectively. There was a significant positive correlation between the number of oocytes and the number of ovarian follicles (r = 0.493, P <0.01) ng / L, but the difference was not statistically significant (P> 0.05). Conclusion With the development of ovarian follicles during ovulation induction, the level of VEGF in serum is significantly increased. With age, the level of VEGF in follicular fluid is significantly increased.