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目的建立结核分枝杆菌杂交信号放大检测方法。方法构建纳米颗粒信号放大载体,建立杂交信号放大方法,检测标本中结核分枝杆菌特异性的插入序列IS6110。应用该方法检测124份临床结核患者标本,并与细菌培养和生化鉴定法的检测结果进行比较,确定该方法的灵敏度和特异性。结果杂交信号放大方法检测临床标本的灵敏度为87.7%,特异性为92.2%,假阳性率为7.8%,假阴性率为12.3%。结论已建立具有较高灵敏度和特异性的杂交信号放大检测方法,该方法操作简便、快速,可作为结核分枝杆菌临床标本的检测方法。
Objective To establish a method to amplify mycobacterium tuberculosis hybridization signal. Methods The nanoparticle signal amplification vector was constructed and a hybridization signal amplification method was established to detect the specific insert of IS6110 in Mycobacterium tuberculosis. The method was used to detect 124 samples of clinical tuberculosis patients and compared with the results of bacterial culture and biochemical identification to determine the sensitivity and specificity of this method. Results The sensitivity of hybridization signal amplification method for detection of clinical specimens was 87.7%, specificity was 92.2%, false positive rate was 7.8%, false negative rate was 12.3%. Conclusion The hybridization signal amplification detection method with high sensitivity and specificity has been established. The method is simple, rapid and can be used as a detection method for clinical specimens of Mycobacterium tuberculosis.