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目的探讨＋ＧＺ作用后，大鼠心肌组织内内皮素－１（ＥＴ－１）和内皮型一氧化氮合酶（ｅＮＯＳ）ｍＲＮＡ表达变化。方法２４只雄性Ｗｉｓｔａｒ大鼠随机分成对照组、＋ＧＺ重复暴露后３０ｍｉｎ、６ｈ和２４ｈ四组，每组６只。对照组大鼠Ｇ值为＋１ＧＺ，实验组大鼠在动物离心机上经历了３次＋１０ＧＺ／３ｍｉｎ（两次间间隔３０ｍｉｎ）作用。分别于暴露后３０ｍｉｎ、６ｈ和２４ｈ处死大鼠取心，提取总ＲＮＡ，用半定量反转录聚合酶链反应（ＲＴ－ＰＣＲ）检测方法检测大鼠心肌组织ＥＴ－１和ｅＮＯＳｍＲＮＡ表达水平。结果＋ＧＺ暴露后３０ｍｉｎ和６ｈ大鼠心肌组织ＥＴ－１ｍＲＮＡ明显升高，ｅＮＯＳｍＲＮＡ明显降低，但２４ｈ时均恢复正常。结论＋ＧＺ暴露可使大鼠心肌组织内ＥＴ－１和ｅＮＯＳｍＲＮＡ表达发生改变，其在心肌损伤中可能起一定的作用，但这种变化是可逆的。
Objective To investigate the expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) mRNA in myocardium of rats after + GZ treatment. Methods Twenty-four male Wistar rats were randomly divided into control group, GZ + GZ group after repeated exposure for 30min, 6h and 24h. The G value of the control group was + 1GZ, and the rats in the experimental group underwent 3 times + 10GZ / 3min in the animal centrifuge (the interval was 30min between two times). The rats were sacrificed at 30 min, 6 h and 24 h after exposure, respectively. The total RNA was extracted and the expression of ET-1 and eNOS mRNA in myocardium was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Results After 30 min and 6 h of GZ exposure, ET-1 mRNA in myocardium was significantly increased and eNOS mRNA was significantly decreased, all of which returned to normal at 24 h. Conclusions + GZ exposure can change the expression of ET-1 and eNOS mRNA in rat myocardium, which may play a role in myocardial injury, but this change is reversible.