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目的 从肝癌患者外周血培养扩增树突状细胞，观察其形态、表型、内吞及递呈抗原能力，为树突状细胞在肝癌生物治疗中的应用奠定基础。方法 从肝癌患者外周血中分离单个核细胞，在 Ｇ Ｍ Ｃ Ｓ Ｆ、 Ｉ Ｌ４ 的诱导下培养扩增树突状细胞。光学显微镜下观察其体外培养过程中的形态特征及变化，电镜观察其超微结构， Ｆ Ａ Ｃ Ｓ观察其表型特征， Ｍ Ｌ Ｒ检测其抗原递呈能力， Ｈ Ｒ Ｐ内吞实验检测其群体内吞能力。结果 肝癌患者外周血树突状细胞具典型的形态及表型特征，较巨噬细胞更强的激发同种混合淋巴细胞反应的能力。其群体的内吞能力约在培养第 ５ 天达高峰，之后有明显下降。结论 从肝癌患者外周血中可获取较大量的、高纯度的、具较强抗原递呈能力的树突状细胞，在其具有活跃的内吞能力时，以合适的肿瘤抗原致敏可望成为肝癌生物治疗的崭新手段。
Objective To investigate the morphology, phenotype, endocytosis and antigen presentation ability of dendritic cells from peripheral blood of patients with hepatocellular carcinoma, and to lay a foundation for the application of dendritic cells in the biological treatment of liver cancer. Methods Mononuclear cells were isolated from the peripheral blood of patients with hepatocellular carcinoma. Dendritic cells were cultured and expanded with the induction of G M C S F and I L 4 . The morphological characteristics and changes during in vitro culture were observed under an optical microscope. Ultrastructure was observed by electron microscopy. The phenotypic characteristics were observed by F A C S, and the antigen-presenting ability was detected by M L R . Mass endocytosis. Results The dendritic cells in peripheral blood of patients with hepatocellular carcinoma had typical morphological and phenotypic characteristics, and they were more able to stimulate the same mixed lymphocyte reaction than macrophages. The endocytotic capacity of its population peaked around the fifth day of culture and then decreased significantly. Conclusion From the peripheral blood of patients with hepatocellular carcinoma, a large number of highly purified dendritic cells with high antigen presentation ability can be obtained. When they have active endocytic capacity, sensitization with suitable tumor antigens is expected to become A new approach to biological treatment of liver cancer.