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利用ＡＰＡＧＥ、荧光原位杂交技术和ＲＦＬＰ标记，对导入黑麦（ＳｅｃａｌｅｃｅｒｅａｌｅＬ．）多小穗等性状创制的小麦新种质１０＿Ａ进行了分子标记检测。ＡＰＡＧＥ分析发现，１０＿Ａ与其他１ＲＳ／１ＢＬ易位系一样，含有１ＲＳ的醇溶蛋白标记位点Ｇｌｄ１Ｂ３。以黑麦基因组总ＤＮＡ作探针，用中国春（Ｔｒｉｔｉｃｕｍａｅｓｔｉｖｕｍｃｖ．ＣｈｉｎｅｓｅＳｐｒｉｎｇ）基因组ＤＮＡ作封阻，与１０＿Ａ根尖细胞有丝分裂染色体进行荧光原位杂交。结果表明，黑麦的１ＲＳ易位到１０＿Ａ中。用２５个ＲＦＬＰ探针进行Ｓｏｕｔｈｅｒｎ分析，进一步发现１０＿Ａ的１ＢＳ特异限制性片段发生丢失，代之以黑麦１ＲＳ的特异限制性片段，而位于其他染色体上的特异限制性片段未发生缺失。据此认为，多小穗小麦新种质１０＿Ａ属于１ＲＳ／１ＢＬ易位系。同时还讨论了１０＿Ａ在小麦遗传改良中的利用情况。
APAGE, fluorescence in situ hybridization and RFLP markers were used to detect the molecular marker of wheat germplasm 10_A, which was introduced into many spikelets of Secalecereale L.. APAGE analysis showed that 10_A, like other 1RS / 1BL translocations, contained a gliadin marker site of 1RS Gld1B3. The total DNA of rye genomic DNA was used as a probe, and the genome DNA of Chinese Spring (Triticum albumum Chinese Spring) was used as a probe to perform fluorescence in situ hybridization with 10 A root tip mitotic chromosome. The results showed that 1RS of rye translocated to 10_A. Southern analysis using 25 RFLP probes further revealed that the 1BS-specific restriction fragment of 10_A was lost, replaced by the specific restriction fragment of rye 1RS, while the deletion of the specific restriction fragments on other chromosomes did not occur. Therefore, the new germplasm 10_A of multi-spikelet belongs to the 1RS / 1BL translocation line. The use of 10_A in wheat genetic improvement was also discussed.