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本文报道了一种基于荧光素的锌离子传感器FN1,其在甲醇溶液中显示了对锌离子极高的灵敏度和选择性。随着锌离子的滴加,紫外-可见光谱中最初在371nm处的吸收峰消失,在437nm处出现了一个新峰。当在混合金属离子的甲醇溶液中滴加了相对于二价金属离子的等物质的量浓度的锌离子或相对于一价金属离子0.5倍物质的量浓度的锌离子后,锌离子积分荧光强度增强了近37倍(Φ=0.48,λmax(em)=513nm),估算的锌离子检测限为7μg·L-1。基于紫外-可见光、荧光和质谱的结果,讨论了1∶1的FN1/Zn2+配合物的结合机理。
This paper reports a fluorescence-based zinc ion sensor, FN1, which exhibits very high sensitivity and selectivity to zinc ions in methanol solution. With the addition of zinc ions, the first absorption peak at 371 nm in the UV-visible spectrum disappears and a new peak appears at 437 nm. When zinc ions in a quantitative concentration relative to the divalent metal ions or zinc ions in a 0.5-fold concentration relative to the monovalent metal ions are added dropwise to the mixed metal ion-containing methanol solution, the integrated zinc ion fluorescence intensity (Φ = 0.48, λmax (em) = 513nm). The detection limit of zinc ion was 7μg · L-1. The binding mechanism of the 1: 1 FNl / Zn2 + complex was discussed based on the results of UV-Vis, fluorescence and mass spectrometry.