论文部分内容阅读
Objective: To isolate the phenolic compounds obtained from the dried roots ofPolygonum multiflorum and investigate their pharmacological activities.Methods: The chemical constituents were isolated and purified by combining them with a macroporous resin (DM-8),MCI gel,and Sephadex LH-20 and by performing ODS column chromatography.Their structures were elucidated by 1D and 2D NMR analyses,as well as mass spectrometry.The isolated compounds were evaluated to determine their hepatoprotective and α-glucosidase inhibitory activities in vitro.Results: Two phenolic compounds,namely,polygonimitin E (1) and polygonimitin F (2),were isolated from the dried roots of P.multiflorum.Compound 2 (10 μmol/L) only showed moderate hepatoprotective activity against N-acetyl-p-aminophenol (APAP)-induced HepG2 cell damage.Unfortunately,these two compounds exhibited no α-glucosidase inhibitory activity.Conclusion: Compounds 1 and 2 were new compounds.Compound 2 could be one of the potential hepatoprotective constituents of P.multiflorum.