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目的为了阐明癌基因蛋白异常表达与乙型肝炎病毒（ＨＢＶ）复制的关系。方法用链霉菌素－生物素（ＳＬＡＢ）免疫组织化学染色和酶联免疫吸附试验（ＥＬＩＳＡ）方法，检测了６４例慢性ＨＢＶ感染者肝细胞中Ｃ－ｅｒｂＢ－２Ｐ１８５、ｒａｓＰ２１和Ｐ５３等癌基因蛋白和血清乙型肝炎病毒ｅ抗原（ＨＢｅＡｇ）。结果Ｃ－ｅｒｂＢ－２Ｐ１８５和ｒａｓＰ２１阳性组中，血清ＨＢｅＡｇ的阳性检出率分别为８９４％和８４６％，而阴性组ＨＢｅＡｇ的检出率分别为２０％和４８％，两组差异显著。结论表明癌基因蛋白Ｃ－ｅｒｂＢ－２Ｐ１８５和ｒａｓＰ２１的异常表达与ＨＢＶ复制密切相关。
Objective To elucidate the relationship between abnormal expression of oncogene protein and hepatitis B virus (HBV) replication. Methods The expressions of C-erbB-2P185, rasP21 and P53 oncogene proteins in hepatocytes of 64 patients with chronic HBV infection were detected by streptavidin-biotin (SLAB) immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) And serum hepatitis B virus e antigen (HBeAg). Results The positive rates of HBeAg in C-erbB-2P185 and rasP21 positive groups were 894% and 846% respectively, while the positive rates of HBeAg in negative group were 20% and 48%, respectively Significant. Conclusion The abnormal expression of oncogene proteins C-erbB-2P185 and rasP21 is closely related to HBV replication.