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目的探讨Survivin基因在老年白血病患者白血病细胞中的表达,并分析与预后的关系。方法应用RT-PCR对32例不同时期老年急性白血病(AL)患者及5例慢性淋巴细胞白血病患者的白血病细胞Survivin基因mRNA表达情况进行检测,以10例健康人作为对照。结果 32例老年AL患者白血病细胞Survivin mRNA阳性表达率为71.87%,急性淋巴细胞白血病(ALL)细胞Survivin mRNA表达率为71.42%与急性非淋巴细胞白血病(ANLL)细胞76%的表达率比较差异无统计学意义,二者均高于对照组(χ2=5.90、6.81,P<0.05)。初治和复发AL患者白血病细胞Survivin mRNA阳性表达率分别为78.01%、84.15%,明显高于对照组(χ2=12.61、9.23,P<0.05),老年慢性淋巴细胞白血病患者的白血病细胞中无Survivin表达。结论老年急性白血病的发生、发展可能与Survivin基因的过度表达密切相关。
Objective To investigate the expression of Survivin gene in leukemia cells of elderly patients with leukemia and analyze its relationship with prognosis. Methods The expression of Survivin mRNA was detected by RT-PCR in 32 patients with AL (acute leukemia) and 5 patients with chronic lymphocytic leukemia at different stages. 10 healthy people were used as control. Results The positive rate of Survivin mRNA expression in leukemic cells was 71.87% in 32 elderly AL patients, 71.42% in acute lymphoblastic leukemia (ALL) cells, and 76% in acute non-lymphocytic leukemia (ANLL) Statistical significance, both higher than the control group (χ2 = 5.90,6.81, P <0.05). The positive rates of Survivin mRNA in the leukemic cells of newly diagnosed and recurrent AL patients were 78.01% and 84.15%, respectively, which were significantly higher than those of the control group (χ2 = 12.61,9.23, P <0.05). There was no Survivin in the leukemia cells of elderly patients with chronic lymphocytic leukemia expression. Conclusion The occurrence and development of senile acute leukemia may be closely related to the overexpression of Survivin gene.