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采用盆栽方法,设置土壤相对含水量(RSM)为85%(对照)、65%、45%、25%,研究干旱胁迫对设施马铃薯全生育期叶片脱落酸(ABA)含量和水分利用效率(WUE)的影响。结果表明:马铃薯发棵期土壤相对含水量在25%~85%范围内,随着土壤水分胁迫强度的增加,马铃薯叶片ABA含量和St NCEDs(9-cis-epoxycarotenoid dioxygenase,9-顺式环氧类胡萝卜素双加氧酶)基因的转录水平呈逐渐增加趋势,重度土壤水分胁迫情况下叶片ABA含量增加33%,St NCED1基因转录提高4倍,而St NCED2基因转录提高9倍。在水分胁迫下马铃薯发棵期叶片水分状况发生不同变化。其中气孔导度呈下降趋势,在土壤相对含水量为45%时下降幅度最大,为81%,并且在同一时间内,土壤相对含水量为45%气孔开放最大;在不同的水分处理间,土壤相对含水量为25%的叶气温差最大,平均比对照高2℃;相反,随着土壤水分胁迫增加,叶水势和叶片相对含水量越来越小,日变化过程中在同一时刻,土壤相对含水量为45%时水分利用效率最高,在12∶00和18∶00达到了最高峰,分别为1.13μmol/mmol和1.15μmol/mmol。
The relative soil water content (RSM) was set as 85% (control), 65%, 45%, 25% by potting method. The effects of drought stress on abscisic acid content and water use efficiency )Impact. The results showed that the relative soil water content of potato was 25% ~ 85% at the first planting stage. With the increase of soil water stress, ABA content in potato leaves and St NCEDs (9-cis-epoxycarotenoid dioxygenase, The transcription level of carotenoid dioxygenase was gradually increased. Under severe soil water stress, ABA content increased 33%, St NCED1 gene transcription increased 4 times, St NCED2 gene transcription increased 9 times. Under water stress, the leaf water status of potato plants changed under different conditions. The stomatal conductance showed a downward trend, with the largest decrease of 81% when the relative water content of soil was 45%, and the maximum of 45% of the soil water content was open at the same time. Under different water treatments, On the contrary, with the increase of soil water stress, the relative water content of leaf water potential and leaf blade became smaller and smaller. During the diurnal variation process, the soil temperature was relatively The highest water use efficiency was obtained at 45% water content, reaching the highest at 12:00 and 18:00, respectively, at 1.13 μmol / mmol and 1.15 μmol / mmol, respectively.