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HBeAg在血清中的浓度很低,难以提纯定量的HBeAg免疫动物,以制备多隆抗血清用于免疫诊断试验。本文报告了将HBeAg和HBsAg阳性血浆经人抗-HBe IgG亲和层析技、Bio-Gel P2柱及蔗糖密度梯度离心,可获得高纯度HBeAg,将其免疫小鼠后经杂交瘤技术得到5H12C81和12H7C161两株杂交瘤,其分泌的单克隆抗体(McAb)均属IgG2a亚型,但结合力研究显示,12H7C161抗体与HBeAg的结合率高于5H12C81McAb和多克隆抗-HBe。作者将这两个单克隆抗-HBe与以前分离
The concentration of HBeAg in serum is so low that it is difficult to purify quantitative HBeAg-immunized animals to prepare a polyclonal antiserum for use in an immunodiagnosis test. This paper reports HBeAg and HBsAg positive plasma by high-purity anti-HBe affinity chromatography, Bio-Gel P2 column and sucrose density gradient centrifugation, to obtain high purity HBeAg, the mice were immunized after hybridoma technology 5H12C81 And 12H7C161 two hybridomas secreted monoclonal antibodies (McAb) belong to IgG2a subtype, but binding studies showed that 12H7C161 antibody and HBeAg binding rate higher than 5H12C81McAb and polyclonal anti-HBe. The authors isolated the two monoclonal anti-HBe from the previous one