移动抑制试验毛细管法的几点修正

• 作 者: 胡乃丰
• 机 构: 贵阳医学院微生物学教研室,
• 出 处: 贵阳医学院学报
• 发表日期: 1989年03期
• 关键词: 毛细管法 移动抑制试验 诱导剂 免疫功能 细胞免疫 细胞悬液 技术措施 培养液 腹腔巨噬细胞 可靠性

巨噬细胞移动抑制试验是检测细胞免疫功能重要指标之一,现已有许多实验方法,其中以毛细管法所得的结果较稳定,且易于观察,特别是用于教学实验中,更能表达这一重要免疫功能现象。在执行这一试验时,需用特制的培养小室,选用合适的腹腔渗出细胞诱导剂,装管和培养方法的可靠性,都需要结合实验室具体条件方能完成。为了使这些技术措施易于执行,故对现行方法作了以下几点修改;经过几年来的教学实验应用,效果较为满意。现

Macrophage migration inhibition test is one of the important indicators to detect cellular immune function, there are many experimental methods, of which the results obtained by the capillary method is more stable and easy to observe, especially for teaching experiments, to better express this Important immune function phenomenon. In the implementation of this test, the need to use a special culture chamber, choose the appropriate peritoneal exudate cell inducing agent, tube and culture of the reliability of the method, both need to be combined with the specific laboratory conditions to be completed. In order to make these technical measures easy to implement, the existing methods are modified as follows. After several years of teaching experiment, the effect is satisfactory. Now