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目的研究琐琐葡萄总黄酮(Flavones from Vitis viniferal L,VTF)对Aβ25-35诱导PC12细胞Bax表达的影响。方法体外培养PC12细胞,分为5组,对照组正常培养,模型组加入20μmol·L-1Aβ25-35作用24h建立阿尔茨海默病(Alzheimer’s disease,AD)细胞模型,实验组分别加入20,40,80mg·L-1浓度VTF干预24h,通过检测细胞活性、乳酸脱氢酶(LDH)释放,观察细胞损伤情况,采用实时荧光定量PCR(Real-time PCR)检测Bax mRNA表达,Western bolt检测Bax蛋白表达。结果模型组较对照组细胞活性降低,LDH释放增加(P<0.01),Aβ25-35诱导PC12细胞明显损伤,VTF实验组与模型组比较,细胞活性增加,LDH释放减少,有显著差异(P<0.05);与模型组比较,给药组Bax表达下降(P<0.01)。结论 VTF通过调节Bax表达,对Aβ25-35诱导的细胞损伤有保护作用。
Objective To investigate the effect of Flavones from Vitis viniferal L (VTF) on Bax expression in PC12 cells induced by Aβ25-35. Methods PC12 cells were cultured in vitro and divided into 5 groups. The control group was cultured normally. Alzheimer’s disease (AD) cell model was established by adding 20μmol·L-1Aβ25-35 to the model group. The experimental groups were given 20,40 , And 80mg · L-1 VTF for 24 h. Cell viability and lactate dehydrogenase (LDH) release were measured to observe the cell injury. Real-time PCR was used to detect the expression of Bax mRNA. Western blot was used to detect Bax Protein. Results Compared with the control group, the cell viability was decreased and the release of LDH was increased (P <0.01). Aβ25-35 induced significant damage to PC12 cells. Compared with the model group, the cell viability increased and the release of LDH decreased in VTF experimental group (P < 0.05). Compared with the model group, the expression of Bax decreased (P <0.01). Conclusion VTF can protect Aβ25-35-induced cell injury by regulating Bax expression.