论文部分内容阅读
目的研究红芪多糖(HPS)对糖尿病心肌病(DC)db/db小鼠心肌组织中过氧化物酶体增值物激活受体γ(PPAR-γ)及葡萄糖转运蛋白4(GLu T4)在心肌组织表达的影响。方法根据体重大小将6周龄的雄性db/db小鼠随机分为5组:模型组(0.9%Na Cl 0.2 m L·d~(-1))、对照组(罗格列酮,4 mg·kg~(-1)·d~(-1))、大中小3个剂量(HPS 200,100,50 mg·kg~(-1)·d~(-1))实验组;正常组(0.9%Na Cl0.2 m L·d~(-1))为同周龄同背景非转基因db/m小鼠12只。连续灌胃8周。于给药前及给药后第2,4,6,8周末,检测小鼠血糖浓度;第8周末处死小鼠,心脏取血并分离血清。用生化法检测血脂及心肌组织中超氧化物歧化酶(SOD)与还原型谷胱甘肽(GSH-PX)活性及丙二醛(MDA)含量;用反转录聚合酶链式反应、蛋白质印迹法检测心肌组织PPAR-γ及Glu T-4 mRNA和蛋白的表达。结果给药8周后,与模型组的血糖为(23.17±2.55)mmol·L~(-1)、三酰甘油(TG)为(5.78±0.50)mmol·L~(-1)、总胆固醇(TC)为(5.93±0.60)mmol·L~(-1)比较,高、中2个剂量实验组和对照组的血糖分别为(16.49±3.64),(17.80±4.40),(16.76±3.25),mmol·L~(-1);这3组的TG分别为(1.59±0.43),(4.02±0.54),(1.12±0.32)mmol·L~(-1);这3组的TC分别为(2.77±0.40),(4.65±0.58),(4.85±0.48)mmol·L~(-1),差异均有统计学意义(P<0.05或P<0.01)。与模型组的SOD为(140.70±1.04)mg·m L~(-1)、GSH-PX为(110.91±0.82)U·mg~(-1)、MDA为(7.20±0.49)nmol·mg~(-1)比较,这3组的SOD分别为(145.81±0.99),(142.21±1.09),(145.70±1.10)mg·m L~(-1),活性显著增强;这3组的GSH-PX分别为(114.94±0.78),(112.10±0.86),(114.84±0.86)U·mg~(-1),活性显著增强;这3组的MDA分别为(5.82±0.52),(6.62±0.67),(5.80±0.52)nmol·mg~(-1),含量显著下降,差异均有统计学意义(P<0.05或P<0.01)。与模型组的PPAR-γmRNA和PPAR-γ蛋白表达分别为0.34±0.11,0.75±0.12、GLu T4 mRNA和GLu T4蛋白表达分别为0.26±0.01,0.42±0.02比较,3个剂量实验组和对照组的PPAR-γmRNA和PPAR-γ蛋白表达分别为0.76±0.06,0.56±0.08,0.45±0.08,0.79±0.10;1.78±0.08,1.44±0.07,1.07±0.05,1.63±0.02。这4组的GLu T4 mRNA和GLu T4蛋白表达分别为0.68±0.05,0.48±0.03,0.49±0.03,0.93±0.05;0.61±0.01,0.45±0.02,0.42±0.01,0.59±0.01,差异均有统计学意义(P<0.01),尤以高剂量组为显著。结论 HPS可降低db/db小鼠的血糖、血脂,同时上调PPAR-γ及Glu T-4的表达,从而改善模型鼠糖、脂代谢紊乱,减缓糖尿病心肌病的病程进展。
Objective To investigate the effect of HsPS on the expression of peroxisome proliferator activated receptor γ (PPAR-γ) and glucose transporter 4 (GLu T4) in myocardium of diabetic cardiomyopathy (db / db) The impact of tissue expression. Methods Six-week-old male db / db mice were randomly divided into 5 groups according to body weight: model group (0.9% NaCl 0.2 m L · d -1), control group (rosiglitazone 4 mg Kg · d -1 · d -1), 3 groups (HPS 200, 100, 50 and 50 mg · kg -1 · d -1), normal group (0.9% Na Cl0.2 m L · d -1) were 12 non-transgenic db / m mice with the same age and background. Continuous gavage for 8 weeks. At the end of the 2nd, 4th, 6th and 8th week after the administration, the blood glucose level of the mice was measured. At the end of the 8th week, the mice were sacrificed and the heart was taken for blood and serum was separated. The activities of superoxide dismutase (SOD) and reduced glutathione (GSH-PX) and the content of malondialdehyde (MDA) in blood lipids and myocardial tissue were detected by biochemical methods. The mRNA expression of MDA was detected by reverse transcriptase-polymerase chain reaction Method to detect the expression of PPAR-γ and Glu T-4 mRNA and protein in myocardial tissue. Results After 8 weeks of treatment, the blood glucose in the model group was (23.17 ± 2.55) mmol·L -1, the triglyceride TG was 5.78 ± 0.50 mmol·L -1, the total cholesterol (TC) was (5.93 ± 0.60) mmol·L -1. The blood glucose of experimental group and control group were (16.49 ± 3.64), (17.80 ± 4.40) and (16.76 ± 3.25 (1.59 ± 0.43), (4.02 ± 0.54) and (1.12 ± 0.32) mmol·L -1, respectively. The TCs of these three groups were respectively (2.77 ± 0.40), (4.65 ± 0.58) and (4.85 ± 0.48) mmol·L -1, respectively. The differences were statistically significant (P <0.05 or P <0.01). Compared with model group, SOD was (140.70 ± 1.04) mg · m L -1, GSH-PX was (110.91 ± 0.82) U · mg -1 and MDA was (7.20 ± 0.49) nmol · mg ~ (-1). The SOD of the three groups were (145.81 ± 0.99), (142.21 ± 1.09), (145.70 ± 1.10) mg · m L -1, respectively. The activity of GSH- PX of (114.94 ± 0.78), (112.10 ± 0.86) and (114.84 ± 0.86) U · mg ~ (-1), respectively. The activities of MDA in the three groups were significantly higher than that of the control group (5.82 ± 0.52, 6.62 ± 0.67 ) And (5.80 ± 0.52) nmol · mg ~ (-1), respectively, the difference was statistically significant (P <0.05 or P <0.01). Compared with model group, the expression of PPAR-γmRNA and PPAR-γprotein were 0.34 ± 0.11,0.75 ± 0.12, GLu T4 mRNA and GLu T4 protein expression was 0.26 ± 0.01,0.42 ± 0.02, respectively. Three doses of experimental group and control group The expression of PPAR-γmRNA and PPAR-γprotein were 0.76 ± 0.06,0.56 ± 0.08,0.45 ± 0.08,0.79 ± 0.10; 1.78 ± 0.08,1.44 ± 0.07,1.07 ± 0.05,1.63 ± 0.02, respectively. The expressions of GLu T4 mRNA and GLu T4 protein in these four groups were 0.68 ± 0.05, 0.48 ± 0.03, 0.49 ± 0.03, 0.93 ± 0.05, 0.61 ± 0.01, 0.45 ± 0.02, 0.42 ± 0.01, 0.59 ± 0.01, respectively Significance (P <0.01), especially in high-dose group was significant. Conclusion HPS can reduce blood glucose and lipids in db / db mice and up-regulate the expression of PPAR-γ and Glu T-4 at the same time, so as to improve the disorder of glucose and lipid metabolism and alleviate the progression of diabetic cardiomyopathy.